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pYLCRISPR/Cas9P35S-H

Plasmid

#66189

Purpose

expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), hygro selection

Depositor

Yao-Guang Liu

Publication

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

cauliflower mosaic virus 35S promoter (P35S)

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#106435

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Publication

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Publication

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

FKBP-EGFP-HOTag3

Expression

Mammalian

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmGFP-P2A-K(AAG)20-P2A-RFP

Plasmid

#105689

Purpose

Double fluorescent stalling reporter K(AAG)20

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRVL-5

Plasmid

#104583

Purpose

Contains human IgG1 CH1/CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Full Fc-mediated immune effector functions

Depositor

Daniel Christ

Publication

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human IgG1 CH1/CH2/CH3 (IGHG1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Publication

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWS171

Plasmid

#90518

Purpose

Cas9 gap repair vector - LEU2

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMXs-mGCN2-FLAG-IP

Plasmid

#101794

Purpose

Retroviral vector encoding C-terminally FLAG tagged mouse GCN2

Depositor

Seiichi Oyadomari

Publication

Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

Insert

Gcn2 (Eif2ak4 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Promoter

LTR

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SaBE4

Plasmid

#100805

Purpose

Expresses SaBE4 in mammalian cells

Depositor

David Liu

Publication

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

SaBE4

Expression

Mammalian

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-DsRed_IRES_SNCA:EGFP

Plasmid

#92195

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for α-Syn screen

Depositor

Huda Zoghbi

Publication

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-SNCA-EGFP

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAAV-hSyn1-mTurquoise2

Plasmid

#99125

Purpose

An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mEGFP-PI35P2

Plasmid

#92419

Purpose

Fluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.

Depositor

Geert van den Bogaart

Publication

Baranov et al Small GTPases. 2017 May 10:0. doi: 10.10

Insert

MCOLN1 (Mcoln1 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Synthetic gene, codon optimized for human.

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Publication

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCDNA5-Flag-BRD4-BD

Plasmid

#90005

Purpose

expresses BD mutant BRD4

Depositor

Kornelia Polyak

Publication

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 140N to 140A, 433N to 433A

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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piggyBac CMV-GFP-FRT

Plasmid

#92078

Purpose

production of transgenic animal and recombination using flipase

Depositor

Jae Yong Han

Publication

Lee et al FASEB J. 2016 Feb;30(2):555-63. doi: 10.

Insert

CMV-eGFP and TK-Neo between FRT3 and FRT

Use

Piggybac

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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CAG-Blimp1

Plasmid

#73995

Purpose

Plasmid expressing mouse Blimp1

Depositor

Connie Cepko

Publication

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Blimp1 gene (Prdm1 Mouse)

Expression

Mammalian

Promoter

CAG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAIP hIL2 co

Plasmid

#90513

Purpose

Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

IL2 (IL2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

Plasmid

#87259

Purpose

YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

Depositor

David Sibley

Publication

Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

Inserts

YFP-mAID-3HA
HXGPRT

Use

Toxoplasma expression

Tags

3HA and mAID

Mutation

Codon optimized for Toxoplasma gondii expression

Promoter

TgDHFR-TS 463 bp and TgTUB1 378 bp

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCasX1

Plasmid

#87685

Purpose

p15A plasmid expressing CasX locus from Deltaproteobacteria

Depositor

Jennifer Doudna

Publication

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasX1

Expression

Bacterial

Promoter

None

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.E545K

Plasmid

#82881

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NM_006218 (PIK3CA Human)

Use

Gateway Cloning

Mutation

E545K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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