We narrowed to 17,309 results for: ESC

Showing: 9721 - 9740 of 17309 results

Results per page:

Sort results by:

HEK4-53bp-tag-insertion-pegRNA

Plasmid

#227673

Purpose

PegRNA for 53 bp insertion

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

SpCas9 sgRNA scaffold (EPHA3 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-LgBiT-2A-EGFP-Vin-PILATeS

Plasmid

#216761

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS

Tags

EGFP and mCherry

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB602-RBCS-tagRFP

Plasmid

#213868

Purpose

Binary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ichikawa et al Plant Physiol. 2023 Oct 4:kiad528. doi:

Insert

RIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)

Expression

Bacterial and Plant

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-ZFN-hSyn-DIO-PSD95-FingR-Citrine-CCR5TC

Plasmid

#213398

Purpose

transcriptionally regulated, double floxed, postsynaptic marker of PSD95 fused to Citrine

Depositor

Katrin Willig

Article

Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

Insert

FingR for endogenous PSD95, transcriptinally regulated

Use

AAV

Tags

Citrine

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBstSK+IRES-V5-IPpoI

Plasmid

#206321

Purpose

A plasmid that facilitates production of mRNA encoding V5 epitope tagged I-PpoI

Depositor

Brian McStay

Article

van Sluis et al Genes Dev. 2015 Jun 1;29(11):1151-63. do

Insert

IRES element-V5 epitope tag- IPpoI open reading frame

Use

Mrna production

Tags

V5-IPpoI fusion protein

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GB1-ALIX(P801G)

Plasmid

#180025

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(1-868, P801G). P801G mutation facilitates expression in E. coli.

Depositor

Lalit Deshmukh

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

GB1-His6-TEV-ALIX(1-868, P801G) (PDCD6IP Human)

Tags

GB1-His6-TEV

Expression

Bacterial

Mutation

P801G

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK 3xFLAG-MTUS1 zeo

Plasmid

#192323

Purpose

Lentiviral expression vector for an inducible 3xFLAG-MTUS1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

MTUS1 (MTUS1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLIK 3xFLAG-KPNB1 zeo

Plasmid

#192326

Purpose

Lentiviral expression vector for an inducible 3xFLAG-KPNB1

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KPNB1 (KPNB1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEN_TT 3xFLAG-XRCC6

Plasmid

#192302

Purpose

Gateway entry vector for an inducible 3xFLAG-XRCC6

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

XRCC6 (XRCC6 Human)

Use

Gateway Cloning

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPT-mCherry

Plasmid

#190605

Purpose

Obtained by cloning 5 stop codons in place of the EGFP Kozak sequence in pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Inserts

mEGFP
mCherry

Use

Lentiviral

Mutation

EGFP Kozak sequence has been replaced with a stre…

Promoter

EIF1-short

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSN672

Plasmid

#177362

Purpose

Expresses human KIF1A(1-393) fused with leucine zipper and His tag

Depositor

Shinsuke Niwa

Article

Anazawa et al Proc Natl Acad Sci U S A. 2022 Aug 9;119

Insert

KIF1A (KIF1A Human)

Tags

His tag and Leucine zipper

Expression

Bacterial

Promoter

T7

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2362_Tier3(SB)-rtTA-PuroR

Plasmid

#169658

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven rtTA expression and PRPBSA-driven PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFOX6-Tier1-PhCMV-TdTomato

Plasmid

#169515

Purpose

Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven dimeric red fluorescent protein TdTomato

Expression

Mammalian

Promoter

PhCMV

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CBh-Alb-mNG

Plasmid

#183466

Purpose

To produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mNeonGreen

Available Since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZ194

Plasmid

#163640

Purpose

rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Article

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

DHB::2mKate2-P2A-H2B::GFP::3xHA (his-58 Synthetic, Nematode)

Use

CRISPR

Tags

2xmKate2 and GFP

Expression

Worm

Promoter

rps-27

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

Plasmid

#180011

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Article

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TVBB C-term-mTurquoise2-Puro

Plasmid

#169224

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2-P2A-Puro at the C-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2-P2A-Puro

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159671

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

ExuL-NLS-hSpCas9-T2A-GFP

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Nsp4 deltaC-EGFP

Plasmid

#165126

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp4 deltaC (ORF1ab codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p alpha-actinin:GAL4/UAS:TdTomato

Plasmid

#161712

Purpose

Express TdTomato through amplification of GAL4/UAS system

Depositor

Hollis Cline

Article

Hiramoto et al Proc Natl Acad Sci U S A. 2014 Nov 25;11

Insert

TdTomato, GAL4

Use

Xenopus laevis expression

Tags

TdTomato

Expression

Mammalian

Promoter

actin-GAL4, UAS

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge