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pcDNA3.2 mir-1-1 reporter mir16-1.1

Plasmid

#46680

Purpose

Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1.1 (MIR16-1 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Mutation

see sequence and publication

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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GalT-RpHLuorin2

Plasmid

#171719

Purpose

Trans-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-SF3B1-WT

Plasmid

#82576

Purpose

Expresses a codon-optimized ORF of human SF3B1 (wild-type)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-WT (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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MGAT2-RpHLuorin2

Plasmid

#171718

Purpose

Cis-/medial-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-T_CDC45-HaloTag

Plasmid

#244244

Purpose

CRISPR/Cas9 donor plasmid to tag human CDC45 with Halo tag

Depositor

Kazuhiro Maeshima

Article

Minami et al Sci Adv. 2025 Mar 28;11(13):eadu8400. do

Insert

CDC45 (CDC45 Human)

Use

CRISPR

Tags

HaloTag

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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MOM603

Plasmid

#133242

Purpose

expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

Depositor

Shinsuke Niwa

Article

Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

Insert

kif1a (KIF1A Human)

Tags

StrepII tag and mScarlet

Expression

Insect

Promoter

polyhedrin promoter

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B Q78L

Plasmid

#89448

Purpose

Expression of GFP-tagged human Rab27B Q78L in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B Q78L (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

Q78L

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B T23N

Plasmid

#89450

Purpose

Expression of GFP-tagged human Rab27B T23N in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B T23N (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

T23N

Promoter

CMV

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDragon-Ctgf

Plasmid

#155015

Purpose

For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to connective tissue growth factor in the presence of Cre and (r)tTA activity.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Insert

Ccn2 (Ccn2 Mouse)

Use

Mouse Targeting

Mutation

M24T (please see depositor comments)

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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mARGAna (cassette 1, transient)

Plasmid

#197588

Purpose

Second-generation mammalian acoustic reporter gene derived from Anabaena

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

mARGAna (cassette 1)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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