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pXJ514-pAAV-PB3'IR-CAG-PCP-mScarlet-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254953

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-mScarlet-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-mScarlet-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ515-pAAV-PB3'IR-CAG-PCP-mtagBFP-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254954

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-mtagBFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-mtagBFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ520-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-hU6-gRNA-PB5'IR

Plasmid

#254955

Purpose

AAV piggyBac vector with single gRNA and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pXJ521-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-hU6-gRNA-PB5'IR

Plasmid

#254956

Purpose

AAV piggyBac vector with single gRNA and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ494-pAAV-PB3'IR-CAG-MCP-GFP-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254949

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-GFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ496-pAAV-PB3'IR-CAG-PCP-GFP-KASH-6XPP7-dual sgRNA-PB5'IR

Plasmid

#254950

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven PCP-sfGFP-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

PCP-sfGFP-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ511-pAAV-PB3'IR-CAG-MCP-mScarlet-KASH-6XMS2-dual sgRNA-PB5'IR

Plasmid

#254951

Purpose

AAV piggyBac vector with dual gRNAs and CAG-driven MCP-mScarlet-KASH reporter; optimized for snRNA-seq and sn-multiome Perturb-seq via nuclear-enriched gRNA capture.

Depositor

Xin Jin

Article

Zheng et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.

Insert

MCP-mScarlet-KASH

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 10, 2026

Availability

Academic Institutions and Nonprofits only

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pTW2278

Plasmid

#252579

Purpose

TRV2 multiplex vector expressing wild type Ymu1, AtCHLl1 gRNA4 and AtPDS3 gRNA12

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1 with AtCHLl1 gRNA 4 and AtPDS3 gRNA12

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW2279

Plasmid

#252580

Purpose

TRV2 multiplex vector expressing wild type Ymu1, AtPDS3 gRNA12 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1 with AtPDS3 gRNA 12 and AtCHLl1 gRNA 4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pTW2498

Plasmid

#252581

Purpose

TRV2 multiplex vector expressing wild type Ymu1, AtCHLl1 gRNA6 and AtCHLl1 gRNA4

Depositor

Steven Jacobsen

Article

Weiss et al Plant Biotechnol J. 2026 Mar 17. doi: 10

Insert

Ymu1 with AtCHLl1 gRNA6 and gRNA 4

Use

CRISPR

Expression

Plant

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT3-T2A-Cre

Plasmid

#189008

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT3 transcript variant 1 ORF and Cre recombinase, separated by T2A motifs

Depositor

Bryan Luikart

Article

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT1-T2A-Cre

Plasmid

#189011

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Article

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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rtTA-PuroR, Mito-EGFP-OMM-PINK1-PRKN

Plasmid

#252078

Purpose

A PiggyBac donor vector that allows for the Dox-inducible expression of mito-EGFP, OMM-PINK1, and PRKN for enforced mitophagy in mammalian cells.

Depositor

Jun Wu

Article

Schmitz et al Cell. 2025 Aug 21;188(17):4773-4789.e22.

Insert

Mito-EGFP, OMM-PINK1, PRKN (PINK1, PRKN Human)

Use

Piggybac transposon donor vector

Expression

Mammalian

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ134B-TLS

Plasmid

#245879

Purpose

Golden gate entry vector carrying TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

TLS2

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ132B-PtALSgRNA

Plasmid

#245880

Purpose

Golden gate entry vector carrying the gRNA for base editing in Poplar hybrid ALS gene

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

PtALSgRNA

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ133B-TLS

Plasmid

#245882

Purpose

Golden gate entry vector carrying TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

TLS2

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131-FCY-UPP

Plasmid

#245873

Purpose

Golden gate entry vector carrying expression cassette for genes FCY and UPP

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

FCY1-UPP

Use

CRISPR

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLCV2-CDK12(hU6-sg2-mU6-sg3)-Blast

Plasmid

#208344

Purpose

pLentiCRISPRv2-Blast backbone with two separate sgRNAs against CDK12

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Insert

cyclin dependent kinase 12 (CDK12 Human)

Use

CRISPR and Lentiviral

Promoter

hU6, mU6

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pCR8-CDK12

Plasmid

#208347

Purpose

Gateway cloning entry vector with CDK12. Must be recombined into a DEST vector for expression.

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Insert

cyclin dependent kinase 12 (CDK12 Human)

Use

Gateway: entry vector

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pLCV2-CDK13(hU6-sg2-mU6-sg3)-Blast

Plasmid

#208348

Purpose

pLentiCRISPRv2-Blast backbone with two separate sgRNAs against CDK13

Depositor

Peter S Nelson

Article

Frank et al eLife 2025

Insert

cyclin dependent kinase 13 (CDK13 Human)

Use

CRISPR and Lentiviral

Promoter

hU6, mU6

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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