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pmCherry-PH1(K14A)
Plasmid#86600PurposeExpresses mCherry-tagged PH(K14A) domain of human Akt1DepositorInsertK14A mutant of Akt1 PH domain (AKT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Trib1
Plasmid#131156PurposeMammalian expression of flag-tagged mouse TRIB1DepositorInsertTrib1 (Trib1 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
Plasmid#80272Purposemammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Px458-Cas9n-Trp63-Exon4-2sgRNA
Plasmid#88849PurposeCRISPR KO of Trp63DepositorInsertTrp63 (Trp63 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_AE
Plasmid#66063PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28-At_PRORP1-His
Plasmid#67869Purposebacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tagDepositorInsertPRORP1 (PRORP1 Mustard Weed)
UseTagsHisExpressionBacterialMutationResidues 36-572 (see comments)PromoterT7Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CD86-mApple
Plasmid#133860PurposeMammalian expression of CD86 N-terminally tagged with EGFP and C-terminally tagged with mAppleDepositorInsertCD86 (CD86 Human)
UseTagsEGFP and mAppleExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9GG
Plasmid#131009PurposeBackbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.DepositorInsertsdirect repeat of SpCas9
promoter PJ23119
mRFP expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterBba_R0040 TetRAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(K14A)
Plasmid#86562PurposeExpresses human full-length Akt1(K14A) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationK14A mutation in the PH domainPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgRNA_Dicer_2
Plasmid#68808Purposespecific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation ofDepositorInsertsgRNA mouse Dicer1
UseCRISPR and Mouse TargetingTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMDC32-FIT2
Plasmid#96990PurposeExpress mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS306Sup35R1
Plasmid#17715DepositorInsertpRS306Sup35R1 (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationAn pRS306 based integrating plasmid designed for …PromoterAvailable sinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
UseTags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only
