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Plasmid#221196Purpose601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601DepositorInsert601[insert(+1)TA-rich]
UseUnspecifiedTagsExpressionMutation601 has an additional mutation added 22 bp from d…PromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 linker-RDF (GB2892)
Plasmid#160604PurposeRDF sequence for C-terminal fusion with PhiC31 recombinase, together with a 18-nt linker sequenceDepositorInsertRDF
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0.178
Plasmid#119645PurposeLevel 0 Part. Insertion siteDepositorInsert7942 NS3 Down Flank
UseSynthetic BiologyTagsExpressionMutation4 nucleotide insertion between nt 65 and 66 in NS…PromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.165
Plasmid#119634PurposeLevel 0 Part. Insertion siteDepositorInsert6803 NS1 Up Flank
UseSynthetic BiologyTagsExpressionMutation1 nucleotide insertion between nt 21 and 22 in NS…PromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0.175
Plasmid#119643PurposeLevel 0 Part. Insertion siteDepositorInsert7942 NS2 Up Flank
UseSynthetic BiologyTagsExpressionMutationbp 422 A to T, bp 423 A to T, bp 663 inserted G. …PromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVR2
Plasmid#84719PurposeE. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcriptionDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutation12 nt stretch lacking A's inserted before 5&…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaL_DdCBE 3-4 (left, DddA11-C) - TL1 alphaDdCBE 1 (left, DddA11-C)
Plasmid#221998PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-3/4 left, alpha-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A | DddA: T1413IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-BLM
Plasmid#80070PurposeExpresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)DepositorInsertBLM (BLM Human)
UseTagsGFPExpressionMammalianMutationIf correct ID, the cDNA is from HeLa and has char…PromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
ACPPiso1-V5
Plasmid#113052PurposeExpresses human prostatic acid phosphatase (isoform 1) in mammalian cellsDepositorInsertprostatic acid phosphatase isoform 1 (ACP3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACPPiso2-V5
Plasmid#113053PurposeExpresses human prostatic acid phosphatase (isoform 2) in mammalian cellsDepositorInsertprostatic acid phosphatase isoform 2 (ACP3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S222TAG
Plasmid#68302PurposeN-terminal MBP tagged human MEK1 gene S222TAG for bacterial expressionDepositorInsertMBP-MEK1 S222TAG (MAP2K1 Human, Synthetic)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S218TAG
Plasmid#68301PurposeN-terminal MBP tagged human MEK1 gene S218TAG for bacterial expressionDepositorInsertMBP-MEK1 S218TAG (MAP2K1 Human, Synthetic)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 3xUTR
Plasmid#40758PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert3 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPKm-195
Plasmid#90496PurposepcDNA3 - CMVmin - PhyB - MTAD, expresses PhyB and MTAD, under CMV promoterDepositorInsertPhyB NT-MTAD
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
psicheck2-B4GALT3
Plasmid#203601PurposeReporter plasmid carrying B4GALT3 3'UTRDepositorInsertbeta-1,4-galactosyltransferase 3 (B4GALT3 Human)
UseLuciferaseTagsExpressionMammalianMutationnt 1800-2330 3'UTRPromoterSV40Available sinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
ELL2mRNA+polyA
Plasmid#127256PurposeFor in vitro translation of human ELL2 with 30 nt pA tailDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationTagsExpressionMutationshortens 3'UTR to 40 bp adds A30 tailPromoterT7Available sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 6xUTR
Plasmid#40761PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert6 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 2xUTR
Plasmid#40757PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert2 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 4xUTR
Plasmid#40759PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert4 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
mutpRL-TK 5xUTR
Plasmid#40760PurposeTo evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNADepositorInsert5 copies of the CXCR4 small RNA target site
UseLuciferaseTagsluciferaseExpressionMutationmutated from TAG to CTC at nt 387–389 of the Reni…PromoterTKAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only