-
Plasmid#210267PurposeInducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 D394R siRES (NFATC2IP Synthetic)
UseTagsmCherryExpressionMammalianMutationD394R, siRESPromoterCMV/TetO2Available sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mCherry(Y70A)
Plasmid#209703PurposeMammalian expression of mCherry with Y70A mutationDepositorInsertmCherry(Y70A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
GOSR2(G144W)-mCherry
Plasmid#209887PurposeExpresses human GOSR2 (GS27) G144W-mutant fused to mChery in mammalian cellsDepositorInsertGOSR2 (GOSR2 Human)
UseTagsmCherryExpressionMammalianMutationChanged glycine 144 to tryptophan; this mutation …PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
MCP-mCherry-TIS11B
Plasmid#207670PurposeTo express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11BDepositorInsertTIS11B (ZFP36L1 Human)
UseTagsMCP-mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(7G)-mCherry
Plasmid#206949PurposeMammalian expression of heterobifunctional SasG linkers (7G)DepositorInsertsfGFP-SaSasG (7G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(6G)-mCherry
Plasmid#206948PurposeMammalian expression of heterobifunctional SasG linkers (6G)DepositorInsertsfGFP-SaSasG (6G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(5G)-mCherry
Plasmid#206947PurposeMammalian expression of heterobifunctional SasG linkers (5G)DepositorInsertsfGFP-SaSasG (5G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(4G)-mCherry
Plasmid#206946PurposeMammalian expression of heterobifunctional SasG linkers (4G)DepositorInsertsfGFP-SaSasG (4G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-SasG(2G)-mCherry
Plasmid#206944PurposeMammalian expression of heterobifunctional SasG linkers (2G)DepositorInsertsfGFP-SaSasG (2G)-mCherry
UseTagsmCherry and sfGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry hPGK
Plasmid#206231PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-TP-mCherry-UnaG
Plasmid#203483PurposeFor Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.DepositorInsertPlastid transit peptide fused mCherry-UnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-EGFP-N3-CYB5R3
Plasmid#204569PurposeExpresses mCherry-EGFP-tagged CYB5R3 in mammalian cellsDepositorInsertCYB5R3 (CYB5R3 Human)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWalium Htt19Q-mCherry
Plasmid#201248Purposeallows the expression of the insert in Drosophila melanogasterDepositorInsertHuntingtin (HTT Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PIP4K2C
Plasmid#202736Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mss4-Kina
Plasmid#202739Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmCherry:S. cerevisiae Mss4 (377-756) (MSS4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PIP4K2CD280K
Plasmid#202737Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PIP4K2BD278K
Plasmid#202735Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PIP4K2A
Plasmid#202732Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PIP4K2AD273K
Plasmid#202733Purposeexpresses recruitable fluorescent protein-tagged enzymeDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Mss4D636K-Kina
Plasmid#202740Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmCherry:S. cerevisiae Mss4 (377-756)(D636K) (MSS4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1
Plasmid#197421PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2
Plasmid#197422PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3
Plasmid#197423PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_IRES-mCherry
Plasmid#197427PurposeHomology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5DepositorInsertIRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-mCherry
Plasmid#197429PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPOTv7-g418-mCherry
Plasmid#190261PurposeTrypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene taggingDepositorInsertmCherry
UseFor endogenous gene taggingTagsExpressionMutationPromoterread throughAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-mCherry-HRasCT
Plasmid#186704PurposeEncoding membrane-targeted mCherryDepositorInsertmCherry-HRasCT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-hCMV1-T-V5-GR-2A-H2BmCherry-pA
Plasmid#171501PurposeOverexpression of T(Brachyury)-GRDepositorInsertT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C_P176L-mCherry-FRB
Plasmid#185983PurposeMammalian expression of human KIF1C with P176L mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationP176L - mutation causing HSP (SPG58)PromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-CD86-mEGFP
Plasmid#190748PurposeExpression of CD86 double tagged with mCherry2 (N-term) and mEGFP (C-term) in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmCherry2 and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
membrane-mCherry-SpVasa-C3
Plasmid#185587Purposeto recruit Vasa-C3 to membraneDepositorInsertVasa-C3
UseIn vitro transcription (mrna synthesis)TagsmCherry and membrane targeting sequence from Neur…ExpressionMutationC3 mutantPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpVasa-mCherry-LOV
Plasmid#185585Purposefor photoconversionDepositorInsertVasa
UseIn vitro transcription (mrna synthesis)TagsmCherry-LOVExpressionMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
membrane-mCherry-SpVasa
Plasmid#185586Purposeto recruit Vasa to membraneDepositorInsertVasa
UseIn vitro transcription (mrna synthesis)TagsmCherry, S-Tag and membrane targeting sequence fr…ExpressionMutationPromoterAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 CortW22A mCherry
Plasmid#187268PurposeLenticiral expression of mCherry-Cortactin W22ADepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmcherryExpressionMutationW22APromoter5' LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_AU1.C.NWS_mCherry-NLS
Plasmid#178282PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsAU1.C.NWS and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-mCherry-LaminB1
Plasmid#186348PurposeNuclear membrane-targeted mCherry expression in mammalian cellsDepositorInsertmCherry-LaminB1
UseTagsExpressionMammalianMutationWTPromoterRat EF1αAvailable sinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C_R169W-mCherry-FRB
Plasmid#185984PurposeMammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
UseTagsmCherry and FRBExpressionMammalianMutationR169W - mutation causing HSP (SPG58) and silent m…PromoterAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
SITS-ccdb-mCherry
Plasmid#118753PurposeMultisite gateway destination vector for 3' tagging with mCherry for cell free expression. Parton lab clone FFFDepositorInsertccdb
UseTagsmCherryExpressionMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJET-10-mCherry-R11b
Plasmid#179891PurposeDonor with homologous arms for Rab11b to knock in mCherryDepositorInsertRAB11B, member RAS oncogene family (RAB11B Human)
UseCRISPR; Donor for homologous based knock inTagsmCherryExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLZRS-TrkC-mCherry
Plasmid#182949PurposeTo express the rat TrkC fused in the C-terminus with mCherryDepositorInsertTRk-C-mCherry (Ntrk3 Rat, Synthetic)
UseRetroviral and Synthetic BiologyTagsmCherryExpressionMammalianMutationPromoterMMLVAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only