We narrowed to 2,111 results for: D10

1. pDest17_Gcn4 K5

   Plasmid

   #231866

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 K5

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 K5
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   D103K, D115K, D127K, D133K, E143K

   Promoter

   T7

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDest17_Gcn4 E3

   Plasmid

   #231863

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 E3

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 E3
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   D105E, D118E, D139E

   Promoter

   T7

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest17_Gcn4 R3 W

   Plasmid

   #231876

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 R3 W

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 R3 W
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   D103R, N126W, D133R, K143R

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMOD3-mClover-Sh_ble

   Plasmid

   #120329

   Purpose

   Bacterial mClover promoter trap

   Depositor

   James Kirby

   Article

   Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

   Insert

   mClover
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D103N (see comment below)

   Promoter

   (None)

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9m4

   Plasmid

   #47316

   Purpose

   Cas9 D10A+D839A+H840A+N863A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m4
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCSC-SP-PW-NepX (aka: pBOB-NEPX)

   Plasmid

   #12340

   Depositor

   Inder Verma

   Article

   Marr et al J Mol Neurosci. 2004 . 22(1-2):5-11.

   Insert

   NEP-X (MME Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   E585V (inactive mutant)

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTPGI_dCas9

   Plasmid

   #64279

   Purpose

   encodes yeast-optimized dCas9 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   Yeast-optimized dCas9
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   D10A & H840A relative to S. pyogenes Cas9

   Promoter

   pTPGI

   Available since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. ADAM10_Halo_C_allele

   Plasmid

   #178151

   Purpose

   Donor vector for endogenous tagging of human ADAM10 at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (ADAM10 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMOD3-proD/mClover/nat

    Plasmid

    #120330

    Purpose

    Bacterial mClover labeling

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    mClover
    

    Use

    Tags

    D103N (see comment below)

    Expression

    Bacterial

    Mutation

    Promoter

    (None)

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p116 Mfz10a

    Plasmid

    #11070

    Depositor

    Ramesh Shivdasani

    Article

    Malik et al Biochem J. 2000 Aug 1. 349 Pt 3():829-34

    Insert

    Mfz10a (Fzd10 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG415Gal_Gcn4 K5

    Plasmid

    #232966

    Purpose

    Galactose iduced expression of Gcn4 K+ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 K+
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    D103K, D115K, D127K, D133K, E143K

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG415Gal_Gcn4 E+

    Plasmid

    #232963

    Purpose

    Galactose iduced expression of Gcn4 E+in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 E+
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    D105E, D118E, D139E

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG415Gal_Gcn4 R3

    Plasmid

    #232973

    Purpose

    Galactose iduced expression of Gcn4 R++ in yeast

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 R++
    

    Use

    Tags

    TEV cleavage site

    Expression

    Yeast

    Mutation

    D103R, D133R, K143R

    Promoter

    Gal1

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDest17_Gcn4 Aro3+

    Plasmid

    #231884

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 Aro3+

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Aro3+
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    D103Y, N112T, L113Y, S117F, K140R

    Promoter

    T7

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. ced-4S in pXHA

    Plasmid

    #134970

    Purpose

    ced-4S cDNA cloned into pXHA backbone, used for production of antibodies

    Depositor

    Robert Horvitz

    Article

    Chen et al Science. 2000 Feb 25;287(5457):1485-9. d

    Insert

    ced-4 (ced-4 Nematode)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pS241

    Plasmid

    #139645

    Purpose

    full length ced-4L cDNA

    Depositor

    Robert Horvitz

    Article

    Shaham et al Cell. 1996 Jul 26;86(2):201-8. doi: 10.1

    Insert

    ced-4 (ced-4 Nematode)
    

    Use

    Cloning vector

    Tags

    Expression

    Bacterial

    Mutation

    addition of K212 and R213 compared to NCBI refere…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Expression

    Mutation

    D10A and H840A

    Promoter

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT374

    Plasmid

    #125615

    Purpose

    Expresses evoFERNY-BE4max-NG in mammalian cells

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    evoFERNY-BE4max-NG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H102P D104N

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCyCBE

    Plasmid

    #226505

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

    Depositor

    Weizhong Chen

    Article

    Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

    Inserts

    APOBEC1-Cas9D10A
    sgRNA
    sacB gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge