We narrowed to 1,873 results for: FRT

1. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

   Plasmid

   #175521

   Purpose

   Nuclear expression of HaloTag10 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

   Plasmid

   #187079

   Purpose

   Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum

   Depositor

   Kai Johnsson

   Article

   Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

   Insert

   TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dHaloTag7 = HaloTag7-D106A

   Promoter

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

   Plasmid

   #118273

   Purpose

   pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

   Depositor

   Naoshige Uchida

   Article

   Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   6xHis (N terminal on insert), T7 epitope, and Xpr…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

   Plasmid

   #75470

   Purpose

   Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Has Service

   AAV1

   Insert

   Reversed ChR2(H134R)-mCherry
   

   Use

   AAV and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV hSyn FLEx-FRT hM4Di-mCherry

   Plasmid

   #161576

   Purpose

   Expression of hM4Di-mCherry in a Flp-dependent fashion

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM4Di-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   hSyn

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV hSyn FLEx-FRT hM3Dq-mCherry

   Plasmid

   #161575

   Purpose

   Expression of hM3Dq-mCherry in a Flp-dependent fashion

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   hSyn

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

   Plasmid

   #182926

   Purpose

   Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Type

   Empty backbone

   Use

   Tags

   APEX2 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

   Plasmid

   #182925

   Purpose

   Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Type

   Empty backbone

   Use

   Tags

   APEX2 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

    Plasmid

    #187588

    Purpose

    Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NLS
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

    Plasmid

    #187583

    Purpose

    Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PML (PML Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF5/FRT/DEST-3xHA/mGli3/Flag

    Plasmid

    #51246

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

    Plasmid

    #187579

    Purpose

    Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PSPC1 (PSPC1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

    Plasmid

    #187580

    Purpose

    Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5/FRT/TO-Venus-Flag-Gateway (1124)

    Plasmid

    #40999

    Depositor

    Jonathon Pines

    Article

    Di Fiore et al J Cell Biol. 2010 Aug 23;190(4):501-9.

    Type

    Empty backbone

    Use

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

    Plasmid

    #187576

    Purpose

    Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    LMNA (LMNA Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

    Plasmid

    #187575

    Purpose

    Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SRSF1 (SRSF1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

    Plasmid

    #187574

    Purpose

    Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    RNPS1 (RNPS1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-FRT/TO-3*Flag-APEX2 C-term NPAT

    Plasmid

    #187585

    Purpose

    Express FLAG epitope and APEX2-tagged NPAT fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NPAT (NPAT Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EF1a-FRT-MCS-WPRE-SV40 - GCH15

    Plasmid

    #230822

    Purpose

    AAV cloning vector for Flpo-dependent expression under the EF1a promoter.

    Depositor

    Kimberly Ritola

    Type

    Empty backbone

    Use

    AAV; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge