We narrowed to 17,355 results for: URE

1. pC035 - dLwCas13a-msfGFP

   Plasmid

   #91925

   Purpose

   Expresses catalytically inactive LwCas13a for mammalian RNA targeting

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS-3xHA and NLS-msfGFP

   Expression

   Mammalian

   Mutation

   R488A and R1060A

   Promoter

   EF-1a

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX neo PFKP-GFP

   Plasmid

   #116940

   Purpose

   Expression of PFKP-GFP fusion protein

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   PFKP (PFKP Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.4-GFP-TEV-B55

   Plasmid

   #217378

   Purpose

   expresses GFP tagged B55 in mammalian cells; tev cleavable

   Depositor

   Wolfgang Peti

   Article

   Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

   Insert

   B55 (PPP2R2A Human)
   

   Use

   Tags

   EGFP TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-16b-10His-TEV-MCES_VACCA_D1_D12

   Plasmid

   #203671

   Purpose

   The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

   Depositor

   Stephen Cusack

   Article

   Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

   Insert

   MCES_VACCA_D1_D12
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL2-Skp2 promoter-luciferase

   Plasmid

   #81119

   Purpose

   Reporter activity of SKP2 gene promoter

   Depositor

   Sung Hee Baek

   Article

   Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

   Insert

   SKP2 (Skp2 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   contains the region from 1_kb upstream of transcr…

   Promoter

   SKP2

   Available Since

   Sept. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pLemir9-124

   Plasmid

   #31779

   Depositor

   Jerry Crabtree

   Article

   Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

   Insert

   miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP1673

   Plasmid

   #65769

   Purpose

   Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pProEx-IDE-wt

   Plasmid

   #99014

   Purpose

   Express human Insulin Degrading Enzyme (IDE) in E. coli.

   Depositor

   Wei-Jen Tang

   Article

   Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

   Insert

   insulin degrading enzyme (IDE Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   TRC

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSHS207 - Bacterial expression plasmid for WT SpCas9

   Plasmid

   #101199

   Purpose

   Bacterial expression plasmid for WT SpCas9

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. UPB-stomach (UPB1)

    Plasmid

    #180784

    Purpose

    Unique Projection Barcode (UPB1)-Stomach for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. UPB-lung (UPB2)

    Plasmid

    #180788

    Purpose

    Unique Projection Barcode (UPB2)-Lung for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    humanized ChR2-H134R (partial)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. UPB-colon (UPB4)

    Plasmid

    #180786

    Purpose

    Unique Projection Barcode (UPB4)-Colon for Projection-seq

    Depositor

    Rui Chang

    Article

    Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

    Insert

    hM3Dq ( partial )
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETSUMO2_Bacteroidetes-bGSDM-TEV

    Plasmid

    #222101

    Purpose

    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

    Insert

    Bacteroidetes-bGSDM-TEV ORF
    

    Use

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    TEV protease site replaces residues E242–G248

    Promoter

    T7

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSNRW-Z5-Nluc

    Plasmid

    #174487

    Purpose

    Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

    Depositor

    Richard C. Willson

    Article

    Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

    Insert

    Z5-Nluc
    

    Use

    Tags

    6x Histidine tag (N terminal on insert)

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid

    #83485

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSUPER-retro-puro-tetO-CTR1-shRNA

    Plasmid

    #53180

    Purpose

    Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    CTR1 (SLC31A1 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

17. 10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S

    Plasmid

    #60815

    Purpose

    dCas9 with single cysteine residue (M1C) for site-specific labelling

    Depositor

    Jennifer Doudna

    Article

    O'Connell et al Nature. 2014 Sep 28. doi: 10.1038/nature

    Insert

    dCas9 M1C D10A C80S H840A C574S
    

    Use

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    M1C D10A C80S H840A C574S

    Promoter

    Available Since

    Dec. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

    Plasmid

    #91918

    Purpose

    Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LbfCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTight-9-124

    Plasmid

    #31874

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. 3×Cherry Dam AID Smash

    Plasmid

    #227374

    Purpose

    For inducible expression and cytosolic localization of Dam

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    Dam DNA-adenine methyltransferase
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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