We narrowed to 8,679 results for: sgRNA

1. pLentiCrisprV2 sgRNA hPER2c-term #1

   Plasmid

   #179454

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCrisprV2 sgRNA hPER2c-term #2

   Plasmid

   #179455

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCrisprV2 sgRNA hPER2c-term #3

   Plasmid

   #179456

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV U6-sgRNA UbC-tdTomato

   Plasmid

   #106949

   Purpose

   sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   sgRNA BsmBI/Esp3I cloning site
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-PpU6P-sgRNA-L1L2

   Plasmid

   #113735

   Purpose

   Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-PpU6P-sgRNA-L5L2

   Plasmid

   #113738

   Purpose

   Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv2-TRAF6-targeting sgRNA 2

   Plasmid

   #131346

   Purpose

   gRNA targeting mouse TRAF6

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   gRNA targeting mouse TRAF6 (Traf6 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

   Plasmid

   #129532

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIA4
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-PpU6P-sgRNA-R4R3

   Plasmid

   #113739

   Purpose

   Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-PpU6P-sgRNA-L5L4

    Plasmid

    #113741

    Purpose

    Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-PpU6P-sgRNA-L1R5

    Plasmid

    #113737

    Purpose

    Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    PpU6P::sgRNA
    

    Use

    CRISPR; Gateway entry vector

    Promoter

    P. patens U6 promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLH-sgRNA1-PP7-boxB

    Plasmid

    #75393

    Purpose

    sgRNA1-PP7-boxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XPP7-boxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLH-sgRNA1-MS2-PP7

    Plasmid

    #75392

    Purpose

    sgRNA1-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Expression scaffold for dCas9+sgRNA

    Plasmid

    #171799

    Purpose

    PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Insert

    PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
    

    Use

    CRISPR

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX459-sgRNA2-RPB1-N-term

    Plasmid

    #195111

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA1_Tet-inducible Luciferase reporter

    Plasmid

    #64161

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for Tet-inducible Luciferase reporter
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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