We narrowed to 29,901 results for: des.2

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pJH126

Plasmid

#162482

Purpose

SEC plasmid containing LG1 homology arms and vha-8p::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR, for expression of TIR1 and nuclear BFP reporter in C. elegans.

Depositor

Bob Goldstein

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Use

CRISPR

Tags

vha-8p::TIR1::F2A::AID*::NLS::tbb-2 3'UTR

Expression

Worm

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

Plasmid

#187721

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 349-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-348

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

Plasmid

#187723

Purpose

Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 1-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 419-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

Plasmid

#187708

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-380

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT (492)

Plasmid

#187709

Purpose

Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_FL-CS

Plasmid

#187713

Purpose

Express the catalytically inactive GST-tagged NEDD4L FL in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L FL with C874 mutated to a serine (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

Cys874 mutated to a serine

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)

Plasmid

#187716

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues XX (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-347

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pK18-hACE2

Plasmid

#149449

Purpose

Expresses human ACE2 driven by the K18 promoter

Depositor

Paul McCray

Article

McCray PB et al J Virol. 2007 Jan;81(2):813-21. doi: 10.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Expression

Mammalian

Promoter

K18

Available Since

June 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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TOPO-GOLGA3

Plasmid

#226451

Purpose

For subcloning of human GOLGA3 promoter or for assays using M13 phage

Depositor

Glen Borchert

Article

DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

GOLGA3 (GOLGA3 Human)

Use

Cloning vector

Mutation

No

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_PGK-hACE2

Plasmid

#161612

Purpose

Expression of human ACE2 in mammalian cells

Depositor

Brad Rosenberg

Article

Daniloski et al Cell. 2020 Oct 24. pii: S0092-8674(20)31

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.CD86.C-HA

Plasmid

#154848

Purpose

Expresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Cluster of Differentiation 86 (Cd86 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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EphA2 430-976 pcDNA3.1

Plasmid

#102738

Purpose

Expression of c terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (430-976aa) (Epha2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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p-miR-Report + WT miR-181 BS in CDX2 3UTR

Plasmid

#22792

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Wild-type miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)

Use

Luciferase

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
EphA2 1-427 pcDNA3.1

Plasmid

#102737

Purpose

Expression of N terminal EphA2 in mammalian cells

Depositor

Jin Chen

Article

Amato et al Cancer Res. 2016 Jan 15;76(2):305-18. do

Insert

EphA2 N terminal (1-427aa) (Epha2 Mouse)

Tags

6xHis and myc

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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p-miR-Report + MT miR-181 BS in CDX2 3UTR

Plasmid

#22793

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in Caudal type homeobox transcription factor 2 3 Untranslated Region (CDX2 Human)

Use

Luciferase

Available Since

March 30, 2010

Availability

Academic Institutions and Nonprofits only
pFastBacHTA-NEDD4L_W1-L-W2-HECT

Plasmid

#187724

Purpose

Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 153-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 1-152 and 419-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

Plasmid

#187725

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-380

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

Plasmid

#187726

Purpose

Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-491

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

Plasmid

#187727

Purpose

Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 226-382 removed (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 226-382

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

Plasmid

#187728

Purpose

Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 418-495 removed (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 418-495

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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