162,619 results

1. pAAV-Ptet-RFP-shR-rtTA

   Plasmid

   #35625

   Purpose

   Tet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).

   Depositor

   Howard Gu

   Article

   Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

   Type

   Empty backbone

   Use

   AAV and RNAi; Tet inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ptet

   Available since

   April 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. PJEBAN3

   Plasmid

   #203682

   Purpose

   Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

   Depositor

   Tine Rask Licht

   Article

   Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

   Insert

   Yellow Fluorescent Protein (YFP)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3

   Plasmid

   #32739

   Depositor

   Frank McCormick

   Article

   Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

   Insert

   TCF4E (Tcf7l2 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   The N-terminal deletion form (delta N) lacks amin…

   Promoter

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6M-Pak1 L107F

   Plasmid

   #12212

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Pak1 (PAK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Constitutively active Pak1: L107F

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP(C3)-Nup153

   Plasmid

   #64268

   Purpose

   GFP-tagged mammalian expression of Nup153

   Depositor

   Birthe Fahrenkrog

   Article

   Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

   Insert

   NuP153 (NUP153 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29a-human histone 4-in-One

   Plasmid

   #223237

   Purpose

   Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

   Depositor

   Weixing Zhao

   Inserts

   His6-VSVG-Histone H2A
   Histone H2B
   Histone H3
   Histone H4-TEV-His6-Dual StrepII
   

   Use

   Tags

   Dual StrepII, His6, TEV protease site, and VSVG

   Expression

   Bacterial

   Mutation

   A103G

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2991

   Plasmid

   #144467

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ID2 (ID2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICS Po-ER Long P2A

   Plasmid

   #164113

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Tito Calì, Marisa Brini

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Long P2A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. scAAV-Cre

   Plasmid

   #177933

   Purpose

   Expresses Cre-recombinase and can be packaged into AAV particles.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Insert

   CAG-Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB_EF1a-DjCas13d-msfGFP-Blast

    Plasmid

    #226008

    Purpose

    DjCas13d protein for RNA targeting in piggyBac backbone

    Depositor

    Silvana Konermann

    Article

    Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

    Insert

    DjCas13d RNA-targeting nuclease
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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