We narrowed to 9,978 results for: crispr plasmids

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

Plasmid

#129039

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA11 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

Plasmid

#129030

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA2 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

Plasmid

#129031

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA3 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

Plasmid

#129032

Purpose

targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR

Depositor

Julia K Polansky

Article

Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

Insert

dCas9-huTET1CD, SgRNA4 (huTSDR)

Tags

HA-Tag, NLS and T2A-mCherry

Expression

Mammalian

Mutation

dCas9 (D10A;H840A)

Promoter

CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_Donor/Reporter-COL4A3

Plasmid

#130279

Purpose

COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

Plasmid

#131757

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE#2 Sp/xCas9 gRNA

Use

Gateway Cloning and Synthetic Biology

Promoter

hU6

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

Plasmid

#131756

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE#1 Sp/xCas9 gRNA

Use

Gateway Cloning and Synthetic Biology

Promoter

hU6

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

Plasmid

#131755

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE#1 SaCas9 gRNA

Use

Gateway Cloning and Synthetic Biology

Promoter

hU6

Available Since

Oct. 15, 2020

Availability

Academic Institutions and Nonprofits only

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LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

Plasmid

#131758

Purpose

pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE#2 SaCas9 gRNA

Use

Gateway Cloning and Synthetic Biology

Promoter

hU6

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-NCOA7g3 (BB36)

Plasmid

#139454

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-NCOA7g2 (BB35)

Plasmid

#139453

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMM4_14L

Plasmid

#183063

Purpose

Plasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

Inserts

mCherry
sfpHluorin
BM3R1-HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mCherry and mTurquoise2

Expression

Yeast

Promoter

RET2, Synthetic promoter, and TDH3

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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LLP1021

Plasmid

#239943

Purpose

Renilla luciferase as a Level-0 part (Golden Gate)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Rluc

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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LLP1022

Plasmid

#239944

Purpose

Firefly luciferase plus as a Level-0 part (Golden Gate)

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

Fluc

Use

Synthetic Biology

Tags

PEST on C-terminus of Fluc plus

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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CaTCH BC1

Plasmid

#154195

Purpose

Lentiviral CaTCH barcoding plasmid harboring a single barcode cassette (BC1)

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

CaTCH BC1

Use

Lentiviral; Catch barcode labeling

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgMETTL1-6

Plasmid

#254417

Purpose

Mammalian expression plasmid encoding an sgRNA targeting human METTL1.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Promoter

U6

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgMETTL1-3

Plasmid

#254416

Purpose

Mammalian expression plasmid encoding an sgRNA targeting human METTL1.

Depositor

Rui Su

Article

Ren et al Blood 2025; 146 (Supplement 1): 2539. do

Insert

METTL1 (METTL1 Human)

Use

Lentiviral

Promoter

U6

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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LLP979

Plasmid

#239901

Purpose

CaMV 35S-SynPro-14 engineered promoter driving the expression of Renilla luciferase

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

CaMV 35S-SynPro-14::Rluc

Use

Synthetic Biology

Tags

PEST on C-terminus of Rluc

Mutation

N/A

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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LLP955

Plasmid

#239877

Purpose

TCTP-SynPro-08 engineered promoter driving the expression of Renilla luciferase

Depositor

Ryan Lister

Article

Khan et al Nat Biotechnol. 2025 Mar;43(3):416-430.

Insert

TCTP-SynPro-08::Rluc

Use

Synthetic Biology

Tags

PEST on C-terminus of Rluc

Mutation

N/A

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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