We narrowed to 12,202 results for: 112

1. pETSUMO2_Bradyrhizobium-bGSDM

   Plasmid

   #212126

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Bradyrhizobium bGSDM
   

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

   Plasmid

   #115142

   Purpose

   CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver, Keith Joung

   Article

   Marino et al Science. 2018 Sep 6. pii: science.aau517

   Insert

   human codon optimized Mb3Cas12a
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304-VHLdeltaB-IRES-GFP

   Plasmid

   #187642

   Purpose

   Mutant VHL Expression vector with GFP

   Depositor

   William Kaelin

   Article

   Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

   Insert

   VHL (VHL Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   deletion of amino acids 91 to 121

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-EphB4-Fc

   Plasmid

   #200986

   Purpose

   Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB4 (EPHB4 Human)
   

   Tags

   Fc region of human IgG1

   Expression

   Mammalian

   Mutation

   Soluble ectodomain of EphB4

   Promoter

   CMV

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS1518

   Plasmid

   #205981

   Purpose

   FLAG-SV40 NLS-Cas6(Cse3/CasE) from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas6(Cse3/CasE) from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS1514

   Plasmid

   #205977

   Purpose

   FLAG-SV40 NLS-Cas8/Cse1 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8/Cse1 from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS1515

   Plasmid

   #205978

   Purpose

   FLAG-SV40 NLS-Cas11/Cse2 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas11/Cse2 from MWEE01000013, Cas5-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH4R-P2A-mCherry-N1

   Plasmid

   #84333

   Purpose

   Co-expresses (untagged) Histamine-4 receptor and mCherry

   Depositor

   Dorus Gadella

   Article

   van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

   Insert

   H4R-P2A-mCherry (HRH4 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)

   Plasmid

   #78516

   Purpose

   Lentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   LAT (LAT Human)
   

   Use

   Lentiviral

   Tags

   mCitrine

   Expression

   Mammalian

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC19-E3CY56

    Plasmid

    #103075

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p23-mNeongreen-DDX21 HGV-mRuby3

    Plasmid

    #179227

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mNeongreen and mRuby3

    Expression

    Mammalian

    Mutation

    D338H, E339G

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR2.1-promoter-mIL-6

    Plasmid

    #109296

    Purpose

    IL-6 promoter reporter construct

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Insert

    murine IL-6 promoter (Il6 Mouse)
    

    Expression

    Mammalian

    Mutation

    CMV promoter in pEGFP-N1 replaced with murine IL-…

    Promoter

    murine IL-6 promoter

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTRAP-bio

    Plasmid

    #68530

    Purpose

    Expresses full-length P. vivax MTRAP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

    Depositor

    Gavin Wright, Julian Rayner

    Article

    Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

    Insert

    MTRAP
    

    Tags

    rat CD4 d3+4, enzymatic biotinylation sequence

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-BG505.DS-SOSIP.4mut-gp160

    Plasmid

    #123277

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.4mut variant

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-6xHis-GFP(+36)-DL4

    Plasmid

    #199168

    Purpose

    used to express a supercharged GFP variant that forms inclusion bodies in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+36)-DL4
    

    Tags

    MGHHHHHHGGA

    Mutation

    E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

    Promoter

    T7

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCEP4-BG505.DS-SOSIP.6mut-gp160

    Plasmid

    #123278

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variant

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-E6MBW6

    Plasmid

    #103102

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-SOCS6-WT

    Plasmid

    #74496

    Purpose

    Overexpression of SOCS6 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

    Insert

    SOCS6 (Socs6 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    unknown

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pMW76: pET-His-MBP-SUMO-R2Bm-33XTEN- SpCas9(H840A)-SII

    Plasmid

    #209292

    Purpose

    Expresses R2Bm-Cas9(H840A) fusion in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Tags

    His, MBP, SpCas9(H840A), StrepII, and bdSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. PHKA1 gRNA (BRDN0001144977)

    Plasmid

    #77838

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PHKA1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PHKA1 (PHKA1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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