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pMax_mPlum_C207G/T208C

Plasmid

#177831

Purpose

Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mPlum_C207G/T208C

Expression

Mammalian

Mutation

C207G + T208C

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Nanog-CreER targeting construct

Plasmid

#59720

Purpose

Cre-ER knockin targeting construct for mouse endogenous Nanog locus

Depositor

Jacob Hanna

Article

Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

Insert

creERT2

Use

Mouse Targeting

Promoter

mNanog

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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gBeta Cerulean

Plasmid

#27810

Depositor

Steven Vogel

Article

Thaler et al Biophys J. 2005 Oct . 89(4):2736-49.

Insert

mCerulean C1-Gbeta

Expression

Mammalian

Available Since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pSB305-K3L-vac

Plasmid

#20053

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBS Wnt-5bHA

Plasmid

#17377

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-5bHA (Wnt5b Mouse)

Tags

HA

Expression

Bacterial

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pLenti-CamKIIα-CRY2PHR-P2A-CIBN-mCherry-VAMP2

Plasmid

#178574

Purpose

Lentiviral vector for CamKIIα::Opto-vTrap

Depositor

Won Do Heo

Article

Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

Insert

CRY2PHR-P2A-CIBN-mCherry-VAMP2

Use

Lentiviral

Promoter

CaMKIIa

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-AncMEcoGII291

Plasmid

#205024

Purpose

Expressing engineered M.EcoGII with higher thermostability and DNA methylation activity

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 291

Tags

His6x tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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TfR-mCh-DHFR

Plasmid

#173013

Purpose

For light regulated release of transferrin receptor (TfR) from the endoplasmic reticulum. Driven by CMV promoter.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

TfR-mCh-DHFR

Tags

DHFR and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1149 - intron 3 smu-2 PATCs

Plasmid

#161516

Purpose

PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron 3 from smu-2

Expression

Worm

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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AY42_pS.Donor.R5

Plasmid

#100291

Purpose

CCR5-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

18 bp RFLP (MluI+KspAI)

Use

CRISPR and Synthetic Biology; Human targeting

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Smad2-3'UTR

Plasmid

#61775

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3 Ub-UBB+1

Plasmid

#23967

Depositor

Nico Dantuma

Article

Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

Insert

Ubiquitin B +1 transcript (UBB Human)

Expression

Mammalian

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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pCnRed

Plasmid

#232908

Purpose

Electroporation vector for expression of the lambda Red recombineering system in Cupriavidus necator.

Depositor

Anita Emmerstorfer-Augustin

Article

Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

Insert

gam (gam Synthetic, phage Lambda)

Use

Synthetic Biology

Promoter

Pbad-rha

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11

Plasmid

#221183

Purpose

cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Expression

Bacterial

Promoter

PQ5 (cumate-inducible)

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ConVERGD-(HA)hM4Di-W3SL

Plasmid

#218759

Purpose

Provides AND intersectional (Cre+Flp) expression of HA-hM4Di

Depositor

Lindsay Schwarz

Article

Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

Insert

hM4Di

Use

AAV

Tags

HA

Promoter

hSyn

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCAMBgfp

Plasmid

#206130

Purpose

This binary vector has been generated for introduction of SGFP in filamentous fungi using ATMT. Contains the hygromycin resistance gene and the SGFP gene.

Depositor

Anne Osbourn

Article

Sesma et al Nature. 2004 Sep 30;431(7008):582-6. doi

Insert

hygromycin resistance gene and the Promoter of ToxA+SGFP

Use

Binary vector for expression of sgfp in filamento…

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2991

Plasmid

#144467

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ID2 (ID2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1330

Plasmid

#143950

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TFAP2C (TFAP2C Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3196

Plasmid

#144672

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZIC1 (ZIC1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-RAB5A

Plasmid

#176488

Purpose

For lentiviral transduction of 3FLAG-RAB5A

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

RAB5A (RAB5A Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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