164,629 results

1. pCDNA3-DCX-TurboID-V5

   Plasmid

   #190740

   Purpose

   Mammalian expression of DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   doublecortin (DCX Human)
   

   Use

   Tags

   TurboID and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti-sgKeap1#1/Cre

   Plasmid

   #173639

   Purpose

   Expresses a Keap1-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Keap1 (Keap1 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX2T-TEV CHMP1B 1-143

   Plasmid

   #108286

   Purpose

   Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

   Depositor

   Wesley Sundquist

   Article

   Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

   Insert

   CHMP1B (CHMP1B Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-143

   Promoter

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pM-TagRFP657

   Plasmid

   #176563

   Purpose

   Expression of TagRFP657 for auxotrophic selection in the absence of methionine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   TagRFP657
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pZBTB4-donor

   Plasmid

   #176374

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZBTB4 (ZBTB4 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDM326_DPAKa(GBD)-YFP

   Plasmid

   #128701

   Purpose

   expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

   Insert

   GBD domain (aa 731-890) of DPAKa
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP

   Expression

   Mutation

   none

   Promoter

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

   Plasmid

   #111923

   Purpose

   Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_19μ
   

   Use

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   E67D, F92W, E104D, D133E at CaM

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET23b-15F11-HA-mEGFP-6xHis

   Plasmid

   #129593

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV-EF1amCherry-2A-OsTIR1-W

   Plasmid

   #159296

   Purpose

   Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   OsTIR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti6.2-ccdB-Nanoluc

    Plasmid

    #87075

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge