We narrowed to 30,860 results for: LIS;

1. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

   Plasmid

   #51509

   Purpose

   Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV1

   Insert

   tdTomato-T2A-SypEGFP
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1-spikeRBD::∆ACE2 SURF

   Plasmid

   #206957

   Purpose

   Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

   Depositor

   Xiaokun Shu

   Article

   Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

   Inserts

   sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
   ACE2 (ACE2 Human)
   

   Tags

   cSURF, mCherry, and nSURF

   Expression

   Mammalian

   Mutation

   17-615 and RBD (319-541)

   Promoter

   CMV

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPRv2_RB1#2

   Plasmid

   #174151

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   RB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-SpCas9(KWRQLC)-P2A-EGFP (RAS2864)

   Plasmid

   #223087

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KWRQLC amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KWRQLC)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(KWRQLC)=D1135K, S1136W, G1218R, E1219Q, R1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

   Plasmid

   #223114

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CL20_mEGFP_CUX1_NUTM1

   Plasmid

   #205800

   Purpose

   Express mEGFP-tagged fusion protein, CUX1_NUTM1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   CUX1_NUTM1 (CUX1, NUTM1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmiR-report-MYB-WT

   Plasmid

   #141114

   Purpose

   Reporter plasmid of WT MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   CMYB (MYB Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmiR-report-MYB-mut

   Plasmid

   #141115

   Purpose

   Reporter plasmid of mutant MYB in mammalian cells.

   Depositor

   Jianjun Chen

   Article

   Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

   Insert

   MYB (MYB Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   A to T mutations at m6A sites

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-3_Flag-YTHDF2 (nonsense mutation of sgYTHDF2-1)

   Plasmid

   #232945

   Purpose

   Expresses YTHDF2 with synonymous mutations rendering it resistant to sgYTHDF2-1-mediated knockdown

   Depositor

   Jianjun Chen

   Article

   Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Lentiviral

   Mutation

   synonymous mutation

   Available Since

   May 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. GCN5 core-dCas9-p300 core

    Plasmid

    #179553

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PCDH-METTL14-R298P

    Plasmid

    #141113

    Purpose

    Overexpression of METTL14 mutant in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    HMETTL14 (METTL14 Human)
    

    Expression

    Mammalian

    Mutation

    Substitution - Missense, position 298, R➞P

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_BCL9L_CBL

    Plasmid

    #205779

    Purpose

    Express mEGFP-tagged fusion protein, BCL9L_CBL from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    BCL9L_CBL (BCL9L, CBL Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-3_Flag-YTHDF2-m6A-MUT

    Plasmid

    #238976

    Purpose

    Expresses the m6A-binding-deficient mutant of YTHDF2

    Depositor

    Jianjun Chen

    Article

    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.

    Insert

    YTHDF2-m6A-MUT (YTHDF2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    2 site mutation (W432A+W486A)

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-SpCas9(LWKYQS)-P2A-EGFP (AHK34)

    Plasmid

    #223079

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(LWKYQS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(LWKYQS)=D1135L, S1136W, G1218K, E1219Y, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. p300 core-dCas9-CBP core

    Plasmid

    #179559

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human p300 core (aa 1048-1664) & c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, EP300 Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmiRA1-MYC

    Plasmid

    #141121

    Purpose

    Lentiviral overexpression of MYC in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid

    #223115

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid

    #223116

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLED.NPL

    Plasmid

    #193014

    Purpose

    Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR

    Plasmid

    #67277

    Purpose

    inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.

    Depositor

    Jannik Elverløv-Jakobsen

    Article

    Callesen et al Mol Oncol. 2017 Nov;11(11):1616-1629. do

    Inserts

    first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
    second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
    

    Tags

    mTQ2 blue fluorescent and red flourescent gene ka…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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