Skip to main content
Addgene
Showing: 161 - 200 of 518 results

  1. pCAG-hPPP3CB-FLAG

    Plasmid
    #179135
    Purpose
    Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11
    Insert
    protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179135-plasmid-map-sequence-id-352807

    Enlarge

  5. pRegev_V2

    Plasmid
    #204144
    Purpose
    Single reporter randomly selected from splicing library for biochemical validation.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    V2 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204144-plasmid-map-sequence-id-405477

    Enlarge

  6. pRegev_V3

    Plasmid
    #204145
    Purpose
    Single reporter randomly selected from splicing library for biochemical validation
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    V3 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204145-plasmid-map-sequence-id-405464

    Enlarge

  7. pRegev_S1.46G>U

    Plasmid
    #204160
    Purpose
    pRegev_S1 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    S1 exon with 46G>U substitution in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    46G>U substitution
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204160-plasmid-map-sequence-id-405475

    Enlarge

  8. pRegev_S2.39U>G

    Plasmid
    #204163
    Purpose
    pRegev_S2 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S2 with 39U>G mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    39U>G mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204163-plasmid-map-sequence-id-405473

    Enlarge

  10. pRegev_S4.51A>C

    Plasmid
    #204172
    Purpose
    pRegev_S4 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S4 with 51A>C mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    51A>C mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204172-plasmid-map-sequence-id-405448

    Enlarge

  11. pRegev_S4.39U>G

    Plasmid
    #204171
    Purpose
    pRegev_S4 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S4 with 39U>G mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    39U>G mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204171-plasmid-map-sequence-id-405446

    Enlarge

  12. pRegev_D1

    Plasmid
    #204178
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon D1 in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204178-plasmid-map-sequence-id-405445

    Enlarge

  14. pRegev_S5.49C>A

    Plasmid
    #204175
    Purpose
    pRegev_S5 with single mutation that disrupts secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S5 with 49C>A mutation in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    49C>A mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204175-plasmid-map-sequence-id-405426

    Enlarge

  15. pRegev_D4

    Plasmid
    #204184
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    D4 exon in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204184-plasmid-map-sequence-id-405407

    Enlarge

  16. pRegev_D2

    Plasmid
    #204180
    Purpose
    Single reporter with long guanine depleted (G-poor) region from splicing library.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon D2 in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    61C>G mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    204180-plasmid-map-sequence-id-405406

    Enlarge

  19. pMADM-alpha

    Plasmid
    #36890
    Depositor
    Liqun Luo
    Article
    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma
    Inserts
    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    ATG (T)
    GFP
    Use
    Tags
    3 Myc tags
    Expression
    Mammalian
    Mutation
    deleted nucleotides after nucleotide 274, deleted…
    Promoter
    CAG (chicken beta actin promoter and CMV enhancer…
    Available since
    Aug. 17, 2012
    Availability
    Academic Institutions and Nonprofits only
    36890-plasmid-map-sequence-id-46195

    Enlarge

  20. pRegev_S4.39U>G.51A>C

    Plasmid
    #204173
    Purpose
    pRegev_S4 with double mutations that restore secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S4 with 39U>G and 51A>C mutations in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    39U>G and 51A>C mutations
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204173-plasmid-map-sequence-id-405470

    Enlarge

  21. pRegev_S1.34C>A_46G>U

    Plasmid
    #204161
    Purpose
    pRegev_S1 with double mutations that restore secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    S1 exon with 34C>A and 46G>U substitutions in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    34C>A and 46G>U mutations
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204161-plasmid-map-sequence-id-405452

    Enlarge

  22. pRegev_S2.39U>G.51A>C

    Plasmid
    #204165
    Purpose
    pRegev_S2 with double mutations that restore secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S2 with 39U>G and 51A>C mutations in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    39U>G and 51A>C mutations
    Promoter
    Truncated CAG
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204165-plasmid-map-sequence-id-405443

    Enlarge

  23. pRegev_S5.49C>A.59G>U

    Plasmid
    #204177
    Purpose
    pRegev_S5 with double mutations that restore secondary structure.
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    Exon S5 with 49C>A and 59G>U mutations in beta globin splicing minigene
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    49C>A and 59G>U mutations
    Promoter
    Truncated CAG
    Available since
    Aug. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    204177-plasmid-map-sequence-id-404686

    Enlarge

  29. UAS:bloSwitch

    Plasmid
    #108355
    Purpose
    UAS driven green to red switch reporter for B3 recombinase expression
    Depositor
    Harold Burgess
    Article
    Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.
    Insert
    UAS:[Ubiquitin-C-intron]-[blown-out site]-nuclear-GFP-[beta-globin-polyA]-[blown out site]-[membrane TagRFPT]
    Use
    Zebrafish expression
    Tags
    Expression
    Mutation
    Zebrafish codon optimized
    Promoter
    lac
    Available since
    Sept. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    108355-plasmid-map-sequence-id-208272

    Enlarge

Showing: 161 - 200 of 518 results