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Showing: 161 - 200 of 509 results

  3. pCL21-decoder

    Plasmid
    #159448
    Purpose
    Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pact1-lexA-ER-B112-phosphodegron-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    act1
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159448-plasmid-map-sequence-id-313196

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  4. pCL28-decoder

    Plasmid
    #159450
    Purpose
    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    PlexAtet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PlexAtet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159450-plasmid-map-sequence-id-313198

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  5. pCL33-decoder

    Plasmid
    #159451
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1mut
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159451-plasmid-map-sequence-id-313201

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  6. pCL38-decoder

    Plasmid
    #159452
    Purpose
    Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1tet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1tet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159452-plasmid-map-sequence-id-313203

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  7. pCL66-decoder

    Plasmid
    #159453
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1mut
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159453-plasmid-map-sequence-id-313208

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  8. pCL12-decoder

    Plasmid
    #159447
    Purpose
    Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    PlexAtet-Citrine-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PlexAtet
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159447-plasmid-map-sequence-id-313193

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  12. LT2_14_GlyOx

    Plasmid
    #177711
    Purpose
    Plasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)
    Depositor
    Lisbeth Olsson
    Article
    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d
    Inserts
    pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
    pTEFmut8-mCherry-tADH1
    pTRX2_5xUAS-ymYPET-tCYC1
    Use
    Used as donor dna in genome integration after lin…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    177711-plasmid-map-sequence-id-378782

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  13. pJO629

    Plasmid
    #104342
    Purpose
    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2
    Insert
    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    Use
    Tags
    3xFLAG
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    104342-plasmid-map-sequence-id-207903

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  14. pACCS3-1

    Plasmid
    #78559
    Purpose
    4.7 kb HindIII ETEC CS3 fimbrial operon from pCS100 cloned into pACYC184.
    Depositor
    James Kaper
    Article
    Giron et al Vaccine. 1995 Jul;13(10):939-46.
    Insert
    cs3 operon
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tet
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. natMX-ins7

    Plasmid
    #195044
    Purpose
    pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference
    Depositor
    Gloria Brar
    Article
    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75
    Insert
    NatR
    Use
    Yeast genomic targeting
    Tags
    tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    195044-plasmid-map-sequence-id-384248

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  27. pGG431

    Plasmid
    #165615
    Purpose
    Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165615-plasmid-map-sequence-id-330960

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  28. pGG442

    Plasmid
    #165616
    Purpose
    Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
    Use
    CRISPR
    Tags
    3x HA, SV40 NLS, Zif268, and c-Myc-like NLS
    Expression
    Yeast
    Mutation
    Corrected homology relative to wild type SpCas9 (…
    Promoter
    TEF1
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165616-plasmid-map-sequence-id-330958

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  29. pKP864

    Plasmid
    #167658
    Purpose
    Express colicinB-His6
    Depositor
    Kathleen Postle
    Insert
    cba-his6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167658-plasmid-map-sequence-id-337304

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  30. pKP968

    Plasmid
    #167653
    Purpose
    Express colicinB-His6, D17C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 D17C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with D17C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167653-plasmid-map-sequence-id-339836

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  31. pKP996

    Plasmid
    #167654
    Purpose
    Express colicinB-His6, T18C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 T18C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with T18C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167654-plasmid-map-sequence-id-339834

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  32. pKP995

    Plasmid
    #167655
    Purpose
    Express colicinB-His6, M19C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 M19C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with M19C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167655-plasmid-map-sequence-id-339832

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  33. pKP997

    Plasmid
    #167656
    Purpose
    Express colicinB-His6, V20C, M261C
    Depositor
    Kathleen Postle
    Insert
    cba-his6 V20C, M261C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6 with V20C, M261C mutations
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167656-plasmid-map-sequence-id-339829

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  34. pKP2264

    Plasmid
    #167660
    Purpose
    Express colicinB-His6, A121C, Y254C
    Depositor
    Kathleen Postle
    Insert
    cba-his6, A121C, Y254C
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba-his6, I121C, Y254C
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167660-plasmid-map-sequence-id-337305

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  35. pKP579

    Plasmid
    #159598
    Purpose
    Express Cba and Cbi protein
    Depositor
    Kathleen Postle
    Insert
    cba and cbi
    Use
    Tags
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    159598-plasmid-map-sequence-id-313311

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  36. pKP2085

    Plasmid
    #167606
    Purpose
    Express ColicinB-V5-TEV-T7-mature MBP
    Depositor
    Kathleen Postle
    Insert
    cba-malE translational fusion
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cba ORF fused to V5 tag, TEV protease site, T7 ta…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167606-plasmid-map-sequence-id-335991

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  37. pKP1469

    Plasmid
    #160815
    Purpose
    Expression of ExbB protein; the exbB gene replaces tonB gene in BamHI site of pKP325; includes pBAD promoter and optimized RBS from pBAD24-derived pKP660 (ExbB, ExbD)
    Depositor
    Kathleen Postle
    Insert
    exbB
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160815-plasmid-map-sequence-id-316117

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Showing: 161 - 200 of 509 results