We narrowed to 4,920 results for: FU

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RP1B FUS 1-163 QQ4xSS #1

Plasmid

#127194

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #1 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-413aa-V5

Plasmid

#29612

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-413

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA 3.2-FUS-1-269aa-V5

Plasmid

#29610

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-269aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-269

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA 3.2-FUS-1-373aa-V5

Plasmid

#29611

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

V5

Expression

Mammalian

Mutation

Contains FUS aa# 1-373

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
RP1B FUS 1-163 QQ4xSS #2

Plasmid

#127195

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #2 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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TH0994-pOCC177-Fus-wt_(1-211)

Plasmid

#221886

Purpose

Shuttle vector for baculovirus production, using FlashBac bacmid

Depositor

Anthony Hyman

Article

Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

Insert

FUS (FUS Human)

Tags

6xHis-MBP-PreScission and TEV-SNAP-tag

Expression

Insect

Mutation

PLD domain (aa 1-211)

Promoter

PH promoter

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc his Su(fu) (CT#768)

Plasmid

#13855

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Tags

his and myc

Expression

Mammalian

Mutation

full ORF

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

Plasmid

#228400

Purpose

Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

NES/PKI-mNeonGreen2x-NLS/FUS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

Plasmid

#223287

Purpose

A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS

Use

Synthetic Biology; Piggybac transposase insertabl…

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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FUS_anion

Plasmid

#98656

Purpose

Expresses untagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_WT

Plasmid

#98651

Purpose

Expresses MBP-tagged full length FUS

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFUS-WT-VC

Plasmid

#175068

Purpose

expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells

Depositor

Arnold Boersma

Article

Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

Insert

FUS glycine rich protein (FUS Human)

Tags

HA and mVenus + mCherry

Expression

Mammalian

Mutation

mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

Promoter

CMV Promoter

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK-NFLAG-hFUS

Plasmid

#50487

Purpose

Produces lentivirus expressing human FUS with FLAG tag at its N-terminus by co-transfection with psPAX2 and pMD2G

Depositor

Zissimos Mourelatos

Article

Nakaya et al RNA. 2013 Apr;19(4):498-509. doi: 10.126

Insert

FUS (FUS Human)

Use

Lentiviral

Tags

FLAG

Promoter

TRE

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only
pENTR4_FUS

Plasmid

#26366

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FUS (FUS Human)

Use

Entry vector

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
MBP-FUS_LC_WT

Plasmid

#98653

Purpose

Expresses MBP-tagged FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_12E

Plasmid

#98652

Purpose

Expresses MBP-tagged full length phosphomimetic FUS

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_6E

Plasmid

#98655

Purpose

Expresses MBP-tagged full length phosphomimetic FUS

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

6 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_12E

Plasmid

#98654

Purpose

Expresses MBP-tagged phosphomimetic FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR4_FLAG/HA_FUS_His6

Plasmid

#26365

Depositor

Thomas Tuschl

Article

Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

Insert

FUS (FUS Human)

Use

Entry vector

Tags

FLAG/HA and His6

Mutation

delta 527 (stop codon)

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
FUS_Halo_N_allele_400

Plasmid

#161767

Purpose

Donor vector for endogenous tagging of human FUS at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2olig-BFP

Plasmid

#155355

Purpose

Lentivector to express CRY2olig-BFP control

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

cry2olig-BFP

Use

Lentiviral

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_Halo_C_allele_400

Plasmid

#161768

Purpose

Donor vector for endogenous tagging of human FUS at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

halotag (FUS Human)

Use

Donor vector for endogenous tagging

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSupAR-MbPylRS(DiZPK)

Plasmid

#91705

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

Depositor

P. Chen

Article

Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

Inserts

pylS
pylT

Expression

Bacterial

Mutation

T810G, C813T, C820G, T821C, T937A, A1046T, G1257A

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pBS U3-hTR-500

Plasmid

#28170

Depositor

Kathleen Collins

Article

Fu et al Mol Cell. 2003 May . 11(5):1361-72.

Insert

U3-hTR-500 (TERC Human)

Expression

Mammalian

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pmCherry-GFP

Plasmid

#86441

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

mCherry-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

mCherry-sfGFP

Promoter

Plac

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLacZa-GFP

Plasmid

#86440

Purpose

visualization and quantifying the out-of-frame mutations from genome editing

Depositor

Yun-Bo Shi

Article

Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

Insert

LacZalpha-MCS-sfGFP fusion gene

Use

Color visualization in bacteria

Tags

LacZa-sfGFP

Promoter

Plac

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF3-N

Plasmid

#155353

Purpose

Express human YTHDF3(1-385) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF3(1-385)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF1-C2

Plasmid

#155350

Purpose

Express human YTHDF1(360-559) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (360-559)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAPf-hYTHDF1-C1

Plasmid

#155349

Purpose

Express human YTHDF1(264-559) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (262-559)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAPf-hYTHDF2-N

Plasmid

#155352

Purpose

Express human YTHDF2(1-381) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF2(1-381)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSNAPf-hYTHDF1-N

Plasmid

#155351

Purpose

Express human YTHDF1(1-359) with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF1 (1-359)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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FUS_LC_charge_RtoK

Plasmid

#139127

Purpose

express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

Add K and D to FUS LC so it has the same charge p…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS_LC_charge

Plasmid

#139126

Purpose

express FUS LC with an hnRNPA2 LC-like charge patterning

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

FUS (FUS Human)

Expression

Bacterial

Mutation

Add R, K, D to make FUS LC have similar charge pa…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-EF1a-GFP1-10-IRES-Puro

Plasmid

#211467

Purpose

Lentivirus backbone expressing GFP1-10

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHO4d-Cas9

Plasmid

#67881

Purpose

Cas9nlsHis6 Bacterial Expression construct

Depositor

Michael Nonet

Article

Fu et al Nucleic Acids Res. 2014 Dec 16;42(22):13

Insert

Cas9

Use

CRISPR

Tags

His6 and T7 epitope

Expression

Bacterial

Promoter

T7

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pRSET-mEosEM

Plasmid

#132708

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mEosEM

Expression

Bacterial

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211462

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ApaLID211A-HA

Plasmid

#211459

Purpose

Express mitochondrial localized ApaLI with D211A mutation

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-D211A

Tags

HA

Expression

Mammalian

Mutation

D211A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ApaLI*-HA

Plasmid

#211461

Purpose

Express mitochondrial localized ApaLI with D211A, E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI mutant

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-ApaLIE225A, K227A-HA

Plasmid

#211460

Purpose

Express mitochondrial localized ApaLI with E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-E225A-K227A

Tags

HA

Expression

Mammalian

Mutation

E225A, K227A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiCRISPR v2-sgSmg7

Plasmid

#161809

Purpose

guide RNA for Smg7

Depositor

Yi Zhang

Article

Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

Insert

sgRNA targeting mouse Smg7 (Smg7 Synthetic, Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL7368

Plasmid

#217375

Purpose

sfGFP reporter for Bce crcB fluoride riboswitch

Depositor

Julius Lucks

Article

Bushhouse et al bioRxiv 2024.03.29.587317

Insert

Bce crcB riboswitch sfGFP reporter

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL6951

Plasmid

#217373

Purpose

sfGFP reporter for Bsu pbuE* 2AP riboswitch

Depositor

Julius Lucks

Article

Bushhouse et al bioRxiv 2024.03.29.587317

Insert

Pca rhtB riboswitch sfGFP reporter

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJBL730

Plasmid

#217374

Purpose

sfGFP reporter for Bsu yxjA 2AP riboswitch

Depositor

Julius Lucks

Article

Bushhouse et al bioRxiv 2024.03.29.587317

Insert

yxjA riboswitch sfGFP reporter

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Mito-mEosEM

Plasmid

#132706

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

mitochondria targeting sequence

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
mEosEM-Lamin A

Plasmid

#132707

Purpose

application in Correlative light-electron microscopy

Depositor

Pingyong Xu

Article

Fu et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

lamin A

Tags

mEosEM

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

Plasmid

#211463

Purpose

Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-dead

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D211A, E225A, K227A

Promoter

TRE

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-CMV-TFAM-BioID2-IRES-Puro

Plasmid

#211465

Purpose

Lentivirus backbone expressing human TFAM fused with BioID2

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

TFAM-BioID2

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-EF1a-F0ATPMLS-mCherry-GFP1-10

Plasmid

#211466

Purpose

Lentivirus backbone expressing mCherry and GFP1-10 fusion protein with a mitochondrial localization signal

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

mito-mCherry-GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 4,920 results for: FU