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Showing: 201 - 250 of 345 results

  3. pET303-ColE1-TR-E192C-del1-40

    Plasmid
    #180235
    Purpose
    Expresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364) with C terminal cysteine for maleimide chemistry
    Depositor
    Joanna Slusky
    Article
    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:
    Insert
    Colicin E1
    Use
    Tags
    6x His-tag
    Expression
    Bacterial
    Mutation
    Truncation of ColE1 containing T and R domains (r…
    Promoter
    T7
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    180235-plasmid-map-sequence-id-358071

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  6. pFS_0362_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._BA-91_ARRAY_1

    Plasmid
    #116974
    Purpose
    Expresses CaBA-91RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Candidatus Accumulibacter sp. BA-91 RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    116974-plasmid-map-sequence-id-227208

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  7. pFS_0356_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1_RC

    Plasmid
    #116968
    Purpose
    Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1 RC, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    116968-plasmid-map-sequence-id-227033

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  8. pFS_0355_pET30-RT-CRISPR_Candidatus_Accumulibacter_sp._SK-02_ARRAY_1

    Plasmid
    #116967
    Purpose
    Expresses CaSK-02RT-Cas1-Cas2 under pT7lac promoter, encodes CaCRISPR array 1, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Candidatus Accumulibacter sp. SK-02 RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    116967-plasmid-map-sequence-id-227031

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  18. Affibody HER2:243.BirA*-pET301/NT-DEST

    Plasmid
    #174692
    Purpose
    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-HER2 affibody ZHER2:342 fused to the mutated form of BirA enzyme, BirA*
    Depositor
    Jean Gariepy
    Article
    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2
    Insert
    Anti-HER2 affibody ZHER2:342 fused to mutated form of BirA, BirA*
    Use
    Tags
    HIS tag
    Expression
    Bacterial
    Mutation
    R118G mutation in BirA
    Promoter
    T7 promoter
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174692-plasmid-map-sequence-id-347066

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  19. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116955
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    116955-plasmid-map-sequence-id-226973

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  20. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid
    #174691
    Purpose
    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*
    Depositor
    Jean Gariepy
    Article
    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2
    Insert
    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    Use
    Tags
    HIS tag
    Expression
    Bacterial
    Mutation
    R118G mutation in BirA
    Promoter
    T7 promoter
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174691-plasmid-map-sequence-id-347065

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  21. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116956
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    116956-plasmid-map-sequence-id-227069

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  23. pFS_0453_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)_T7term_ARRAY2_FaqI

    Plasmid
    #117006
    Purpose
    Expresses E. coli codon-optimized FsRT-Cas1-Cas2 under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117006-plasmid-map-sequence-id-227036

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  24. pFS_1113_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184741
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23103
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184741-plasmid-map-sequence-id-361527

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  25. pFS_1142_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23103-Leader1-ARRAY1-DR1-FaqI_Term_NotI

    Plasmid
    #184742
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 1 transcribed by BBa_J23103
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184742-plasmid-map-sequence-id-361529

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  26. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184739
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184739-plasmid-map-sequence-id-361530

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  27. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184740
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184740-plasmid-map-sequence-id-361528

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  28. pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

    Plasmid
    #117005
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    117005-plasmid-map-sequence-id-227039

    Enlarge

  29. pFS_1061_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_GGC(BbsI)-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid
    #184738
    Purpose
    encodes aTc inducible FsRT-Cas1–Cas2 and transcriptionally insulated FsCRISPR Array 2
    Depositor
    Randall Platt
    Article
    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.
    Insert
    FsRT-Cas1(opt)-Cas2(opt)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    sequence codon optimized for expression in E. coli
    Promoter
    pTetA
    Available since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184738-plasmid-map-sequence-id-361531

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  33. MFE23-ZZ

    Plasmid
    #223553
    Purpose
    MFE23-ZZ, a bispecific adaptor molecule, was created by fusing a scFv, termed MFE23, recognizing carcinoembryonic antigen to protein ZZ, which binds to the Fc fragment of IgG.
    Depositor
    Jean Gariepy
    Article
    Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6
    Insert
    pET302-MFE23-ZZ
    Use
    Tags
    HA tag and HIS tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223553-plasmid-map-sequence-id-458178

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  34. PDZ3-pET28a

    Plasmid
    #31229
    Depositor
    Andrew Lee
    Insert
    PSD95 PDZ3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2011
    Availability
    Academic Institutions and Nonprofits only

  35. rk16s

    Plasmid
    #117436
    Purpose
    Expression of ARL4
    Depositor
    Richard Kahn
    Insert
    ARL4 (ARL4A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    117436-plasmid-map-sequence-id-226665

    Enlarge

  36. rk4s

    Plasmid
    #117434
    Purpose
    Expression of ARL4a
    Depositor
    Richard Kahn
    Insert
    ARL4a (ARL4A Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117434-plasmid-map-sequence-id-226349

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  37. CS46

    Plasmid
    #117439
    Purpose
    Expression of ARL16
    Depositor
    Richard Kahn
    Insert
    ARL16 (ARL16 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117439-plasmid-map-sequence-id-226442

    Enlarge

  38. ALDO-1_KS

    Plasmid
    #224157
    Purpose
    IPTG-inducible expression of C. elegans protein ALDO-1 with a His tag
    Depositor
    Katherine Walstrom
    Insert
    ALDO-1
    Use
    Tags
    LeuGlu-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    224157-plasmid-map-sequence-id-444332

    Enlarge

  41. rk17s

    Plasmid
    #117437
    Purpose
    Expression of ARL14
    Depositor
    Richard Kahn
    Insert
    ARL14 (ARL14 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    117437-plasmid-map-sequence-id-226358

    Enlarge

  42. pJK615

    Plasmid
    #71699
    Purpose
    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71699-plasmid-map-sequence-id-137034

    Enlarge

  43. rk18s

    Plasmid
    #117438
    Purpose
    Expression of ARL11
    Depositor
    Richard Kahn
    Insert
    ARL11 (ARL11 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    C148R (see comments below)
    Promoter
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    117438-plasmid-map-sequence-id-226383

    Enlarge

  44. rk12s

    Plasmid
    #117435
    Purpose
    Expression of ARL6
    Depositor
    Richard Kahn
    Insert
    ARL6 (ARL6 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117435-plasmid-map-sequence-id-226440

    Enlarge

  45. hFerritinL

    Plasmid
    #122653
    Purpose
    Expression of hFerritinL in bacterial cells
    Depositor
    Trevor Douglas
    Insert
    light chain ferritin, human (FTL Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122653-plasmid-map-sequence-id-237551

    Enlarge

  47. pJL16

    Plasmid
    #117425
    Purpose
    Expression of ARF3
    Depositor
    Richard Kahn
    Article
    et al unpublished
    Insert
    ARF3 (ARF3 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    117425-plasmid-map-sequence-id-228485

    Enlarge

  48. hFerritinH

    Plasmid
    #122652
    Purpose
    Expression of hFerritinH in bacterial cells
    Depositor
    Trevor Douglas
    Insert
    ferritin heavy chain 1 (FTH1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    122652-plasmid-map-sequence-id-237549

    Enlarge

Showing: 201 - 250 of 345 results