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ATF4 8: (SL-)5’ATF4.GFP

Plasmid

#21855

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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ATF4 9: (SL+)5’ATF4.GFP

Plasmid

#21856

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stem loop introduced at the 5' end of the mR…

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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2842 pBS Bcl-2 5'genomic

Plasmid

#8747

Depositor

Stanley Korsmeyer

Article

Veis et al Cell 1993 Oct 22;75(2):229-40.

Insert

Bcl-2 5' genomic (Bcl2 Mouse)

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
ATF4 10: 5’ATF4.uORF1.GFP

Plasmid

#21857

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF1 (uORF) of ATF4 fused to EGFP

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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ATF4 11: 5’ATF4.uORF2.GFP

Plasmid

#21858

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

upstream ORF2 (uORF) of ATF4 fused to EGFP

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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ATF4 14: 5’ATF4.uORF1&2AUA.GFP

Plasmid

#21861

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

AUG codons of uORFs1&2 were converted to AUA

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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ATF4 15: 5’ATF4.uORF1stopm1.GFP

Plasmid

#21862

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stop codon for uORF1 converted to UCG (see author…

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-mScarlet-I(+5)

Plasmid

#219418

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mScarlet-I(+5)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

D125K, D186K, E216K, E238K

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Article

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Insert

35Sp::hCas9

Use

CRISPR

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

Plasmid

#216164

Purpose

Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

Gaussia princeps luciferase (with double mutation to improve stability)

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-PACT 5'-UTR

Plasmid

#115351

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of PACT

Depositor

Ming-Chih Lai

Article

Lai et al FEBS J. 2016 Jan;283(1):88-101. doi: 10.

Insert

PACT 5'-UTR (PRKRA Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

Plasmid

#216318

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

split cerulean fluorescent protein + splice donor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-GFP 5' Half Only

Plasmid

#69907

Purpose

Expresses a circular RNA containing the 5' half of GFP in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

GFP, ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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ATF4 19: (SL)RLuc.5’ATF4.GFP

Plasmid

#21865

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stem Loop upstream of ATF4

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pGL4 mIL-17 2kb promoter + CNS-5

Plasmid

#20128

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-GiDREADD-dTomato-Fishell-5

Plasmid

#83896

Purpose

Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

hM4Di

Use

AAV

Expression

Mammalian

Promoter

hDlx

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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ATF4 17: 5’ATF4.uORF1stopm1&2.GFP

Plasmid

#21863

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Stop codons between the colinear uORF1 and uORF2 …

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pQacR-BCD7-tetR-ssrA P68U18C45T13 v40-5 (569)

Plasmid

#70739

Purpose

plasmid for building 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP

Plasmid

#84256

Purpose

Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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