We narrowed to 616 results for: EN1

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Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

Plasmid

#138460

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-KRAB-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1

Plasmid

#138461

Purpose

lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

MCP-VP64-IRES-zsGreen1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1A

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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PiggyBac-TetO-En1-t2a-Foxa2-hygro

Plasmid

#176483

Purpose

TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

Depositor

Marius Wernig

Article

Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

Insert

En1-t2a-FoxA2 (FOXA2, En1 Mouse, Human)

Expression

Mammalian

Promoter

TetO

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1

Plasmid

#138419

Purpose

CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Insert

dCas9

Use

Lentiviral

Tags

BioTAP-tag

Promoter

EF1a

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pXD80EN12-Pct3-ElVadh(cHis6)-noAccs(DSM2243)

Plasmid

#233881

Purpose

Expresses Eggerthella lenta DSM 2243 El Vadh with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

El Vadh (Elen_0497 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN15-DadhA(cHis8)BC-DadRS-noAccs(ElA2)

Plasmid

#225327

Purpose

Expresses Eggerthella lenta A2 Dadh without accessory genes (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Dadh

Tags

His8

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN10-Pct3-(–)-Eldh1(cHis6)-noAccs(ElA2)

Plasmid

#233880

Purpose

Expresses His-tagged Eggerthella lenta A2 (–)-Eldh1 with a cumate inducible promoter, without accessory genes (inactive), in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eldh1 (Elen_0511 Eggerthella lenta)

Tags

His

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80EN16-HcdhA(cHis8)BC-partial cluster(ElA2)

Plasmid

#225328

Purpose

Expresses Eggerthella lenta A2 Hcdh partial gene cluster (inactive) in Gordonibacter urolithinfaciens DSM 27213

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

Hcdh partial gene cluster

Tags

His8

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)

Plasmid

#225305

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1, (+)-Eldh2

Expression

Bacterial

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

Plasmid

#153062

Purpose

Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Mutagenesis to insert a stop codon at aa 298 in B…

Promoter

EF1α

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

Plasmid

#207135

Purpose

DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)

Expression

Mammalian

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

Plasmid

#207136

Purpose

DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

Plasmid

#138417

Purpose

CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

Depositor

Jian Xu

Article

Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

Inserts

BirA
dCas9

Use

Lentiviral

Tags

FLAG, Avi-tag and V5, His

Promoter

EF1a

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

Plasmid

#108362

Purpose

Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans

Depositor

Luisa Cochella

Article

Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

Inserts

Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
mCherry
his-15 (his-15 Nematode)
unc-54 3' UTR

Tags

2xFlag, MYC, T2A peptide, and TY1 linker

Expression

Worm

Promoter

A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26

Plasmid

#92143

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEN113 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-NeoR-bpA-Frt-Rosa26

Plasmid

#86233

Purpose

Targeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Neomycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

osTir1

Use

Mouse Targeting

Tags

V5 tag

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF1095

Plasmid

#143923

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EN1 (EN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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XE92 XGCNF-EnR

Plasmid

#17029

Depositor

Randall Moon

Article

Song et al Dev Biol. 1999 Sep 1;213(1):170-9. doi:

Insert

XGCNF-EnR (en Fly)

Use

Xenopus expression

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
CAG-EnR

Plasmid

#19715

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

engrailed (en Fly)

Expression

Mammalian

Mutation

Drosophila engrailed repressor domain

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pLIA EnR (CC#29)

Plasmid

#15152

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

Engrailed repressor (en Fly)

Use

Retroviral

Expression

Mammalian

Mutation

engrailed repressor domain

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only