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pLV sgRNA-Notch1 #1 GFP

Plasmid

#106950

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Mutation

intracellular domain *

Promoter

PGK

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
iChr2-Notch Mosaic (SO250)

Plasmid

#99752

Purpose

Second generation Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different chromatin localized fluorescent proteins and Notch signalling genes

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

HIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

Plasmid

#188377

Purpose

Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Low_SynNotch_RHL183

Plasmid

#164827

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Low Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Low Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Promoter

miniCMV and pGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 ΔEMV-6MT

Plasmid

#41737

Depositor

Raphael Kopan, Jeffrey Nye

Article

Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

Insert

Notch-1 Extracellular-domain-deleted construct (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 protein fragment lacking …

Available Since

Jan. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 ICv-6MT

Plasmid

#41730

Depositor

Raphael Kopan

Article

Schroeter et al Nature. 1998 May 28;393(6683):382-6.

Insert

Notch-1 Intracellular Domain (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 protein beginning at Vali…

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107

Plasmid

#64621

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Mutation

intracellular domain *

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pRSFDuet1-His-TEV-OTC_Ub

Plasmid

#172134

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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iMb-Notch-Mosaic (IR99.40)

Plasmid

#99749

Purpose

Rosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different membrane localized fluorescent proteins and Notch signalling genes

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

MbYFP, MbTomato, MbKate2, DN-Rbpj and NICD-PEST

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 Notch1 LNG-6MT

Plasmid

#41738

Depositor

Raphael Kopan, Jeffrey Nye

Article

Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

Insert

Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 leader peptide (aa 1-23) …

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only
pCS2 Notch1 LNG (CC→SS)-6MT

Plasmid

#41739

Depositor

Raphael Kopan

Article

Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

Insert

Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)

Tags

6xMyc

Expression

Mammalian

Mutation

Contains murine Notch-1 leader peptide (aa 1-23) …

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pHR Ef1a_mAbCII-synNotch_tTA-PGK_Puro

Plasmid

#223538

Purpose

Anti-collagen type II scFv-based synNotch; puro-selectable

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Insert

mAbCII_synNotch-tTA

Use

Lentiviral

Tags

c-myc tag

Expression

Mammalian

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

Plasmid

#223536

Purpose

Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Insert

LaG16_synNotch_tTA

Use

Lentiviral

Tags

c-myc tag

Expression

Mammalian

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Notch1 [N253/23R]

Plasmid

#227021

Purpose

Mammalian expression plasmid of anti-Notch1 (Mouse) IgG2a R-mAb. Derived from hybridoma N253/23

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Notch1 (Mus musculus) recombinant mouse monoclonal antibody. (Notch1 Mouse)

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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PGK_ACE2-ECD_Notch-Gal4VP64 (pZYW037)

Plasmid

#194226

Purpose

Lentiviral expression vector expressing ACE2-ECD SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK-ACE2-SynNotch-T2A-mCherry (ACE2 Synthetic)

Use

Lentiviral

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Notch1 [N253/32R]

Plasmid

#206549

Purpose

Mammalian Expression Plasmid of anti-Notch1 (Mouse). Derived from hybridoma N253/32.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Notch1 (Mus musculus) recombinant Mouse monoclonal antibody (Notch1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pKozak_mTagBFP2-T2A-sGFP1-10_synCoTC

Plasmid

#199494

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2-T2A-sGFP1-10

Plasmid

#199497

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2-T2A-sGFP1-10

Use

CRISPR

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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