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Plasmid#109003PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #3
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 1
Plasmid#109002PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #1
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)
Plasmid#217306PurposesgRNA mcherry + PuromycinDepositorInsertpU6-sgRNA, Ef1alpha-Puro-T2A-mcherry
UseCRISPRTagsExpressionMutationPromoterU6, Ef1alphaAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)
Plasmid#217305PurposesgRNA BFP + PuromycinDepositorInsertpU6-sgRNA, Ef1alpha-Puro-T2A-BFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterU6, Ef1alphaAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_PLRKT
Plasmid#111673PurposeMAC-tagged gene expressionDepositorInsertPLRKT (PLGRKT Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
NKP33 GFP (1032)
Plasmid#62022Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertC9orf46 (PLGRKT Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEV-p105-WT
Plasmid#23952DepositorInsertp105 (Nfkb1 Mouse)
UseTagsT7 gp10ExpressionMammalianMutationPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(S536E)
Plasmid#24156DepositorInsertRelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationS536EPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(S276A)
Plasmid#24153DepositorInsertRelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationS276APromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
T7-RelA(S276E)
Plasmid#24154DepositorInsertRelA (RELA Human)
UseTagsT7 gp10ExpressionMammalianMutationS276EPromoterAvailable sinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJH2890
Plasmid#179561PurposePlgc-55B::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVB premotor IN and other neurals expression of miniSOG-SL2 RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagswCherryExpressionWormMutationPromoterPlgc-55BAvailable sinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
hFLT1
Plasmid#83435Purposehuman VEGFR1 with HA and FLAG tagDepositorInserthuman VEGFR1 (FLT1 Human)
UseTagsFlag and MycExpressionMammalianMutationHA-tag was inserted 30 AA downstream of the FLT1 …PromoterCMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL4del
Plasmid#216744PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 4 (OL4) by examining the variant lacking OL4 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL2/4sub
Plasmid#216746PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL3/4sub
Plasmid#216747PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5/4sub
Plasmid#216748PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRP51A+intron-LacZ (pHZ18)
Plasmid#33131DepositorInsertRP51A+intron-LacZ (RPS17A Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pNM261
Plasmid#128411PurposeVanillic acid inducible production of lysostaphin. LsyT with no signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseTagsExpressionBacterialMutationPromoterPspank(V)Available sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNM262
Plasmid#128412PurposeVanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseSynthetic BiologyTagsamyE SPExpressionBacterialMutationPromoterPspank(V)Available sinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only