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We narrowed to 312 results for: SSP

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DSUP_RAMVA_1-208_mCh-SspB (pBS1073)

Plasmid

#185296

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA_1-208

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_47-300_mCh-SspB (pBS1070)

Plasmid

#185293

Purpose

For the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE4_47-300_mCh-SspB (pBS1069)

Plasmid

#185292

Purpose

For the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE2_47-300_mCh-SspB (pBS1068)

Plasmid

#185291

Purpose

For the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE2_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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LLPS2-CAHS2_PARRC::SspB (pBS1043)

Plasmid

#185290

Purpose

For the mammalian expression of the tardigrade protein CAHS2_PARRC attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2_PARRC

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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SspB-mCherry-DHPH-ITSN1

Plasmid

#176103

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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SspB-HaloTag-DHPH1-TRIO

Plasmid

#176115

Purpose

Heterodimerization with iLID, Rac1 and RhoG activation

Depositor

Dorus Gadella

Insert

TRIO (TRIO Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-TRE-SspB-mCh-p60

Plasmid

#190170

Purpose

Doxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

SspBmicro-mCherry-p60

Use

Lentiviral

Tags

SspBmicro-mCherry

Expression

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-80L MBP-SspB Nano

Plasmid

#60409

Purpose

E. coli expression of SspB Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB nano

Use

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

Y11K and A15E

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-SspB-HaloTag-TIAM1-DHPH

Plasmid

#176127

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-SspB-HaloTag-ITSN1-DHPH

Plasmid

#176128

Purpose

Heterodimerization with iLID, Cdc42 activation

Depositor

Dorus Gadella

Article

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

ITSN1 (ITSN1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQE-80L MBP-SspB Micro

Plasmid

#60410

Purpose

E. coli expression of SspB Micro

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

SspB micro

Use

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

R73Q, Y11K and A15E

Promoter

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-43Q

Plasmid

#84352

Purpose

Expression of the human Huntingtin Exon1 protein containing 43Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Use

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHR-pSFFV-TAF15N-mCherry-sspB

Plasmid

#122436

Purpose

Expresses fusion of disordered protein TAF15(1-208), fluorescent protein mCherry, and SspB which upon light activation binds to iLID.

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

mCherry and sspB

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Use

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-29Q

Plasmid

#84350

Purpose

Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Use

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-SspB-HaloTag-TRIO-DHPH1

Plasmid

#176126

Purpose

Heterodimerization with iLID, Rac1 and RhoG activation

Depositor

Dorus Gadella

Insert

TRIO (TRIO Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Httex1-37Q

Plasmid

#84351

Purpose

Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Use

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTWIN1-His6-Ssp-Httex1-15Q

Plasmid

#84348

Purpose

Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon1 (HTT Human)

Use

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
plx208-CMV-SspB-EGFP-VP16

Plasmid

#213532

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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