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RAB_Halo_C_allele

Plasmid

#178124

Purpose

Donor vector for endogenous tagging of human RAB at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (AGFG1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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APP_Halo_C_allele

Plasmid

#178137

Purpose

Donor vector for endogenous tagging of human APP at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (APP Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_v2

Plasmid

#183692

Purpose

Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Expression

Bacterial

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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TREM2_Halo_N_allele

Plasmid

#178122

Purpose

Donor vector for endogenous tagging of human TREM2 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (TREM2 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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GBA_Halo_C_allele

Plasmid

#178127

Purpose

Donor vector for endogenous tagging of human GBA at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (GBA1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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OPTN_Halo_C_allele

Plasmid

#178144

Purpose

Donor vector for endogenous tagging of human OPTN at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (OPTN Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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3XHalo_GFP_TRF1#1

Plasmid

#164644

Purpose

tag TRF1 with GFP and Halo

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

TRF1 (TERF1 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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SP_H6_HaloK73T_KDEL_pCMV1

Plasmid

#183686

Purpose

Mammalian expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Expression

Mammalian

Mutation

K73T in Halotag

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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APOE_Halo_C_allele

Plasmid

#178121

Purpose

Donor vector for endogenous tagging of human APOE at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (APOE Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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DCTN1_Halo_C_allele

Plasmid

#178156

Purpose

Donor vector for endogenous tagging of human DCTN1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (DCTN1 Human)

Use

Donor vector

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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ABCA7_Halo_C_allele

Plasmid

#178150

Purpose

Donor vector for endogenous tagging of human ABCA7 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (ABCA7 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHaloTag-PS

Plasmid

#86626

Purpose

Expresses Akt pseudosubstrate tagged with a HaloTag

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag-PS(RA)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINHALO7

Plasmid

#41117

Depositor

Ray Owens

Article

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV_HaloK73T_cyto

Plasmid

#183687

Purpose

Mammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

Cyto Halo(K73T)

Expression

Mammalian

Mutation

K73T in Halotag

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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TMEM106B_Halo_C_allele

Plasmid

#178173

Purpose

Donor vector for endogenous tagging of human TMEM106B at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (TMEM106B Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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MAPT_Halo_C_allele

Plasmid

#178143

Purpose

Donor vector for endogenous tagging of human MAPT at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (MAPT Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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KIF5A_Halo_C_allele

Plasmid

#178163

Purpose

Donor vector for endogenous tagging of human KIF5A at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (KIF5A Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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PSEN2_Halo_C_allele

Plasmid

#178133

Purpose

Donor vector for endogenous tagging of human PSEN2 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (PSEN2 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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OTUD4_Halo_C_allele

Plasmid

#178164

Purpose

Donor vector for endogenous tagging of human OTUD4 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (OTUD4 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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HNRNPA2B1_Halo_C_allele

Plasmid

#178126

Purpose

Donor vector for endogenous tagging of human HNRNPA2B1 at the C-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (HNRNPA2B1 Human)

Use

Donor vector

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge