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Addgene

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We narrowed to 222 results for: intu

Showing: 81 - 100 of 222 results

  1. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179430
    Purpose
    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-HS4-mch (SH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179430-plasmid-map-sequence-id-355528

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  15. pCL055 AAVS1-9xTetO-UbC-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188747
    Purpose
    Donor vector for integrating UbC promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188747-plasmid-map-sequence-id-371444

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  16. pJT055 AAVS1-BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #187324
    Purpose
    Donor to install a dual surface marker - fluorescence reporter into the AAVS1 safe harbor using HDR
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    BSD-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF1a
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    187324-plasmid-map-sequence-id-369800

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  17. pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry

    Plasmid
    #179431
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-coreHS4-mch (SC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179431-plasmid-map-sequence-id-362554

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  18. pLenti-K36M TAIL[1-44]-3AID-HA-2A-mCherryBSD

    Plasmid
    #225872
    Purpose
    Lentiviral expression of AID degron tagged H3.3(K36M) histone tail corresponding to amino acids 1-44 and mCherry fused BlasticidinR
    Depositor
    Lacra Bintu
    Article
    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.
    Insert
    H3.3(K36M) amino acids 1-44 (H3-3A, H3-3B Synthetic, Human)
    Use
    Lentiviral and Synthetic Biology
    Tags
    3xAID HA and T2A-mCherry
    Expression
    Mammalian
    Mutation
    amino acids 1-44 from H3.3 K36M
    Promoter
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    225872-plasmid-map-sequence-id-449913

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  20. pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #188743
    Purpose
    Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu, Michael Bassik
    Article
    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038
    Insert
    Surface marker and mCitrine reporter
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RSV
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    188743-plasmid-map-sequence-id-371436

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Showing: 81 - 100 of 222 results