Showing: 81 - 100 of 117 results
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Plasmid#197387PurposeTo establish all-in-one TetO-inducible cell line for Pdx1-Mafa expression (Doxycycline induced; rtTA included; Blasticidin resistant)DepositorInsertPDX1-T2A-MAFA
UseLentiviral; TetoTagsExpressionMammalianMutationPromoterTREAvailabilityAcademic Institutions and Nonprofits only -
AD59_pEP.DonorCLYBL.TS
Plasmid#199227PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for PuroR.T2A.EGFP selectable and reporter markers
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailabilityAcademic Institutions and Nonprofits only -
AZ64_pE.DonorCLYBL.TS
Plasmid#199228PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for EGFP reporter
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailabilityAcademic Institutions and Nonprofits only -
BA31_pU.CAG.SaCas9-D10A.rBGpA
Plasmid#199251PurposeExpression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)DepositorInsertSaCas9.D10A nickase
UseCRISPRTags3XHA epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailabilityAcademic Institutions and Nonprofits only -
pVRa29_371
Plasmid#49684DepositorInsertECF29_371
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-ADA 6KR
Plasmid#17561DepositorInsertFoxo1-ADA 6KR (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-ADA 6KQ
Plasmid#17563DepositorInsertFoxo1 ADA 6KQ (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AL08_pgRNA.DMD
Plasmid#100293PurposeDMD intron 43-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6 Polymerase III snRNAAvailabilityAcademic Institutions and Nonprofits only -
AY22_pgRNA.R5.1
Plasmid#100294PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 RNA Pol III promoter human snRNAAvailabilityAcademic Institutions and Nonprofits only -
AY23_pgRNA.R5.2
Plasmid#100295PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6 Polymerase III snRNAAvailabilityAcademic Institutions and Nonprofits only -
BB44_pmc.DonorR5.TS
Plasmid#199223PurposeDonor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locusDepositorInsertExpression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailabilityAcademic Institutions and Nonprofits only -
AM77_pU6.Sa-gRNA.CLYBL
Plasmid#199237PurposeExpression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. aureus gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailabilityAcademic Institutions and Nonprofits only -
AY27_pU6.gRNA.CLYBL
Plasmid#199238PurposeExpression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. pyogenes gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailabilityAcademic Institutions and Nonprofits only -
AP76_pU.CAG.Cas9-D10A-K848A.rBGpA
Plasmid#199253PurposeExpression construct encoding the high specificity SpCas9-KA-D10A nickaseDepositorInsertHigh specificity S. pyogenes Cas9-D10A-KA nickase
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10A K848APromoterCAG promoterAvailabilityAcademic Institutions and Nonprofits only -
AV44_pCAG.Cas9-D10A.gRNA.S1
Plasmid#199258PurposeExpression construct encoding SpCas9-D10A nickase and an AAVS1-targeting guide RNADepositorInsertsSpCas9-D10A nickase
AAVS1-targeting gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationD10APromoterCAG promoter and RNA polymerase III promoter for …AvailabilityAcademic Institutions and Nonprofits only -
AV15_pCAG.Cas9.gRNA.S1
Plasmid#199259PurposeExpression construct encoding SpCas9 nuclease and an AAVS1-targeting guide RNADepositorInsertsSpCas9 nuclease
AAVS1-targeting gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCAG promoter and RNA polymerase III promoter for …AvailabilityAcademic Institutions and Nonprofits only -
AV13_pCAG.Cas9.gRNA.NT
Plasmid#199260PurposeExpression construct encoding SpCas9 nuclease and a control non-targeting guide RNADepositorInsertsSpCas9 nuclease
Non-targeting (NT) control gRNA
UseCRISPRTagsSV40 NLSExpressionMammalianMutationPromoterCAG promoter and RNA polymerase III promoter for …AvailabilityAcademic Institutions and Nonprofits only -
Sema4c-AP-His
Plasmid#72030PurposeExpresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema4c (Sema4c Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Sema5a-AP-His
Plasmid#72035PurposeExpresses the extracellular region of the Sema5A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema5a (Sema5a Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
Sema4c-Fc-His
Plasmid#72156PurposeExpresses the extracellular region of the Sema4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema4c (Sema4c Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 81 - 100 of 117 results