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Showing: 81 - 100 of 12614 results

  1. pLSLGUS

    Plasmid
    #51517
    Purpose
    pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GUS
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Expression
    Mutation
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51517-plasmid-map-sequence-id-78696

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  4. pLentiGuide-BlastR

    Plasmid
    #196970
    Purpose
    Lentiviral acceptor vector for pegRNAs with blasticidin resistance
    Depositor
    Leopold Parts
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    196970-plasmid-map-sequence-id-441210

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  5. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

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  6. pCCG_nit9fl

    Plasmid
    #228409
    Purpose
    Expression of NIT-9 in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228409-plasmid-map-sequence-id-459449

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  7. mCherry_C1_miR21

    Plasmid
    #65970
    Purpose
    Expressed mCherry under the control of miR-21 (miR-21 complementary sequence in 3' UTR)
    Depositor
    Thomas Tuschl
    Insert
    miR-21 complementary sequence
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    65970-plasmid-map-sequence-id-112135

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  8. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid
    #171939
    Purpose
    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein
    Depositor
    David Berson
    Insert
    APEX2-eGFPf
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171939-plasmid-map-sequence-id-338504

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  9. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid
    #171938
    Purpose
    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins
    Depositor
    David Berson
    Insert
    Apex2, mRuby2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171938-plasmid-map-sequence-id-338493

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  10. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid
    #171937
    Purpose
    Expresses APEX2 linked to tau for labeling microtubules
    Depositor
    David Berson
    Insert
    tau-APEX2
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hsyn
    Available since
    July 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    171937-plasmid-map-sequence-id-338490

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  11. pCCG_nit9_without_linker

    Plasmid
    #228411
    Purpose
    Expression of NIT-9 without linkage region in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    deletion of the linkage region
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228411-plasmid-map-sequence-id-459451

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  12. pCCG_nit9fl_reverse

    Plasmid
    #228410
    Purpose
    Expression of NIT-9 with reversed domains in N. crassa at the his-3 locus
    Depositor
    Robert Haensch
    Insert
    NIT-9 domains reversed
    Use
    Protein expression in n. crassa
    Tags
    Expression
    Mutation
    domains reoriented
    Promoter
    Available since
    Jan. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228410-plasmid-map-sequence-id-459450

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  17. sgRNA(MS2)_zeo insert c-MYC

    Plasmid
    #164636
    Purpose
    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64
    Depositor
    Günter Schneider
    Insert
    target sequence for c-Myc activation (MYC Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164636-plasmid-map-sequence-id-326094

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  18. p240 pPr-CREM/Ins

    Plasmid
    #8405
    Purpose
    Androgen-inducible expression of Cre, with chimeric/β-globin intron within the Cre coding sequence and chicken globin insulators.
    Depositor
    Jeffrey Green
    Article
    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56
    Insert
    probasin driven CREM with insulators
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    modified Cre
    Promoter
    Probasin promoter
    Available since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only

  19. pQE80L TriCatcher-RGDSP-MMP

    Plasmid
    #112633
    Purpose
    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher
    Depositor
    Mark Howarth
    Article
    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:
    Insert
    TriCatcher-RGDSP-MMP
    Use
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Mutation
    central matrix metalloproteinase-cleavable sequen…
    Promoter
    T5
    Available since
    Sept. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112633-plasmid-map-sequence-id-222151

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  20. p236 pPr-CREM

    Plasmid
    #8404
    Purpose
    Expression of Cre from tissue-specific, androgen-inducible probasin promoter, with chimeric/β-globin intron within the Cre coding sequence.
    Depositor
    Jeffrey Green
    Article
    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56
    Insert
    probasin driven CREM
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    modified Cre
    Promoter
    Probasin promoter
    Available since
    April 28, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 100 of 12614 results