We narrowed to 784 results for: transfection

1. pEXP1/Joseph2-Multitagged Plasmid

   Plasmid

   #226724

   Purpose

   Validates the expression and detection of epitope-tagged proteins of interest in bacterial cells.

   Depositor

   Richard Cripps

   Article

   Robinson et al Biotechniques. 2024;76(7):299-309. doi:

   Insert

   pEXP1/Joseph2
   

   Use

   Tags

   6xHis, HA, Myc, GFP, Flag, V5

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pT3-EF1α-Dest

   Plasmid

   #133299

   Purpose

   The pT3-EF1α destination vector without any insert which can be used for LR reaction.

   Depositor

   Xin Chen

   Article

   Lee et al Hepatology. 2008 Apr;47(4):1200-10. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTC-ApoE-Tet

   Plasmid

   #85578

   Purpose

   Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver

   Depositor

   Ursula Ehmer

   Article

   Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

   Insert

   CreER
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ApoE.HCR.hAAT

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJRA108

   Plasmid

   #209341

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mCherry fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLCS111

   Plasmid

   #209332

   Purpose

   Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJRA104

   Plasmid

   #209337

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUBQ10-mNeonGreen-GW

   Plasmid

   #209352

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJRA103

   Plasmid

   #209336

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTC-CMV-Tet

   Plasmid

   #85577

   Purpose

   Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liver

   Depositor

   Ursula Ehmer

   Article

   Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

   Insert

   CreER
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ApoE.HCR.hAAT

   Available since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pONSY

    Plasmid

    #111873

    Purpose

    "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807).

    Depositor

    Inaki Ruiz-Trillo

    Article

    Parra-Acero et al Development. 2018 May 11. pii: dev.16210

    Type

    Empty backbone

    Use

    Capsaspora owczarzaki

    Tags

    Expression

    Mutation

    Promoter

    Elongation Factor 1 alpha (EF1a) from Capsaspora

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMH-HA/Joseph2-Multitagged Plasmid

    Plasmid

    #226726

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pMH-HA/Joseph2
    

    Use

    Tags

    HA, Myc, GFP, Flag, V5, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. psgRNA-GST-EGFP-GPIBY55

    Plasmid

    #213720

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. psgRNA-GST-EGFP-GPIDAF

    Plasmid

    #213719

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. psgRNA-GST-EGFP-GPICEAM7

    Plasmid

    #213721

    Purpose

    To express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.

    Depositor

    Qilai Huang

    Article

    Ma et al Front Mol Biosci. 2022 Sep 29;9:1016090.

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    GST

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLCS100

    Plasmid

    #209354

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUBQ10-HALO-GW

    Plasmid

    #209351

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJRA106

    Plasmid

    #209339

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJRA105

    Plasmid

    #209338

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal Dendra2 fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJRA101

    Plasmid

    #209335

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mVenus fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CTMP

    Plasmid

    #129560

    Purpose

    cytoplasmic marker

    Depositor

    Inaki Ruiz-Trillo

    Article

    Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge