We narrowed to 31,661 results for: uros

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EF1a_Puro_Telo_v1

Plasmid

#195138

Purpose

~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Telomere (RTEL1 Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP1

Plasmid

#183323

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP1

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVpuroATT

Plasmid

#47539

Purpose

Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

Depositor

David Weinstock

Article

Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only
CROP_C9_Puro_AAVS1A

Plasmid

#183337

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_AAVS1B

Plasmid

#183338

Purpose

All-in-One CRISPRko system with a guide RNA that targets the AAVS1 locus

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

AAVS1B

Use

Lentiviral

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_C_Myc_DDK_IRES_Puro_TARBP2

Plasmid

#106119

Purpose

Lentiviral expression of TARBP2

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TARBP2 (TARBP2 Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2B

Plasmid

#183325

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2B gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2B

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_TOP2A

Plasmid

#183324

Purpose

All-in-One CRISPRko system with a guide RNA that targets TOP2A gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

TOP2A

Use

Lentiviral

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROG3_P2A_Hygro_Barcode

Plasmid

#120468

Purpose

Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FGFR3

Plasmid

#183291

Purpose

All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FGFR3

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Plasmid

#176310

Purpose

Expresses CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

CreN59_nMag_NLS_P2A_mCherry_IRES_puroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

human EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_Puro_sgAAVS1

Plasmid

#187818

Purpose

pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgAAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_MDS1

Plasmid

#45258

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

MDS1 (MECOM, MDS1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

June 27, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV_puro_41584

Plasmid

#111630

Purpose

Complementation of Dicer_KO mESCs with wild type human DICER isoform

Depositor

Constance Ciaudo

Insert

FLAG-hsDicer (DICER1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

WT

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PHC3

Plasmid

#45262

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PHC3 (PHC3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pT2ADWpuro_2paRAF

Plasmid

#129653

Purpose

For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC2

Plasmid

#183298

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC2

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX_970_puro_DEST_V5_SKIL

Plasmid

#45289

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SNON (SKIL Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
Ii-Str_puromycin

Plasmid

#65309

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Invariant chain (p33)

Expression

Mammalian

Mutation

Streptavidin in its lumenal part

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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Donor_ANKRD1_KO_Puro

Plasmid

#186667

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

ANKRD1 KO Puromycin (ANKRD1 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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