We narrowed to 31,011 results for: uros

1. CDK1as_T2A_puromycin

   Plasmid

   #118595

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROP_C9_Puro_HDAC2

   Plasmid

   #183298

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets HDAC2 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   HDAC2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAX_CAGGS_CAS9_VPR_Puro

   Plasmid

   #167895

   Purpose

   PiggyBac compatible plasmid expressing Cas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Donor_YTHDF2_KO_Puro

   Plasmid

   #186670

   Purpose

   Donor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO Puromycin (YTHDF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pXR003_puroR

   Plasmid

   #219819

   Purpose

   hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe_puro_DEST_Flag_PHC3

   Plasmid

   #45262

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   PHC3 (PHC3 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pbacPuroR

   Plasmid

   #26348

   Depositor

   Ben Lehner

   Article

   Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

   Insert

   Bacterial promoter::PuroR::bacterial 3p flank
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX_970_puro_DEST_V5_SKIL

   Plasmid

   #45289

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   SNON (SKIL Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pAK_DR30_CasRx_Puro

   Plasmid

   #134845

   Purpose

   All in one overexpression of CasRx and guideRNA

   Depositor

   Michael Yaffe

   Insert

   RfxCas13d
   

   Use

   CRISPR

   Tags

   T2A Puromycin

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT2ADWpuro_2paRAF

    Plasmid

    #129653

    Purpose

    For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe_puro_DEST_Flag_MDS1

    Plasmid

    #45258

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MDS1 (MECOM, MDS1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2013

    Availability

    Academic Institutions and Nonprofits only

12. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ii-Str_puromycin

    Plasmid

    #65309

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Streptavidin in its lumenal part

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMyrPalm_mEGFP_IRES_puro2b

    Plasmid

    #21038

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pMyrPalm
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pBabe_puro_DEST_Flag_TNIK

    Plasmid

    #45276

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TNIK (TNIK Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CROP_C9_Puro_DNMT3B

    Plasmid

    #183284

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets DNMT3B gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    DNMT3B
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPL5617_pUG_PuroR

    Plasmid

    #60928

    Purpose

    PCR template vector for generating puromycin resistance cassette.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    puromycin N-Acetyltransferase
    

    Use

    Loxp flanked deletion cassette template

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1_puro_CAG_HexaPro

    Plasmid

    #164077

    Purpose

    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus

    Depositor

    Yannick Doyon

    Article

    Rivest et al protocols.io 2021

    Insert

    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    CRISPR

    Tags

    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CAG

    Available since

    Jan. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Flag_HsIRE1a_deltaP29_D408_pBabePuro

    Plasmid

    #58422

    Purpose

    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    Deleted amino acids P29 to D408

    Promoter

    Available since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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