We narrowed to 687 results for: emd

1. pCJ199

   Plasmid

   #162672

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCJ203

   Plasmid

   #162678

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comomonas thiooxydans (Genbank WP_080747404.1) with deletion of the predicted signal peptide (Phe2:Ala75)

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHETase from Comomonas thiooxydans (Genbank WP_080747404.1) without 75 residue signal peptide
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deltaF2-A75; codon optimized for expression in E.…

   Promoter

   T7/lac

   Available since

   Feb. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET11d-FHis-DND1-Y102A

   Plasmid

   #70082

   Purpose

   Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Use

   Tags

   6xHIS and FLAG

   Expression

   Bacterial

   Mutation

   Y102A. Codon optimized for bacterial expression

   Promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a Cdk2ap1ΔN (MT2B2)-MutTER

   Plasmid

   #178035

   Purpose

   Expression vector for the purpose of protein purification.

   Depositor

   Lin He

   Article

   Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

   Insert

   Cdk2ap1 (Cdk2ap1 Mouse)
   

   Use

   Nonviral

   Tags

   6xHIS - Thrombin - MBP- TEV-TRS

   Expression

   Bacterial

   Mutation

   First N-Terminal 27aa are deleted and Changed T(8…

   Promoter

   T7LacI

   Available since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcI-ts,ind+-(NSP8)

   Plasmid

   #160656

   Purpose

   Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

   Insert

   SARS-CoV-2 NSP8 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pIA1363

   Plasmid

   #166861

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp8 (ORF1ab Synthetic)
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIA1362

   Plasmid

   #166860

   Purpose

   Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp7 (ORF1ab Synthetic)
   

   Use

   Tags

   His-GB1

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sema3a(L, del3)-Fc-His

   Plasmid

   #72136

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Use

   Tags

   Fc-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Sema3a(S)-Fc-His

    Plasmid

    #72138

    Purpose

    Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema3a (Sema3a Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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