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RGS-6xHis-DRE-1-pcDNA3.1-

Plasmid

#52517

Purpose

expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Dre-1 (dre-1 Nematode)

Tags

RGS-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pPD95.75-cYAP-1

Plasmid

#66857

Purpose

Generate transgenic C.elegans with cYAP-1 fused to GFP

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

C.elegans YAP-1 (yap-1 Nematode)

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

C. elegans 5'UTR

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pPD95.75-cYAP-1 S104A

Plasmid

#66858

Purpose

Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pOX-unc103

Plasmid

#16213

Depositor

Larry Salkoff

Insert

eag-like K+ channels (unc-103 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
U653-HTR

Plasmid

#24470

Depositor

Ron Vale

Article

Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

Insert

Unc104 (unc-104 Nematode)

Tags

His

Expression

Bacterial

Available Since

June 2, 2010

Availability

Academic Institutions and Nonprofits only
pCM5.49

Plasmid

#21391

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pal-1 3'utr in pDONRP2RP3

Use

Gateway

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pSR21

Plasmid

#69156

Purpose

read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
TagBFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

rps-27

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pEG608

Plasmid

#40194

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (RNAi resistant) (mex-5 Nematode)

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

Expression

Worm

Mutation

Exon 2 recoded to be RNAi-resistant

Promoter

4.4 kb mex-5 promoter fragment

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pCM3.08

Plasmid

#19405

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

pie-1 ORF+3' in pDONR201 (pie-1 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pGC91

Plasmid

#19621

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC114

Plasmid

#19622

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Prpl-28<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC231

Plasmid

#19644

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pGC78

Plasmid

#19654

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

<1xNLS-lacZ::unc-54(3’)

Use

Flp/frt

Tags

1xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCM3.09

Plasmid

#19406

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

fbf-2 ORF+3' in pDONR201 (fbf-2 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNAOX-twk5

Plasmid

#16105

Depositor

Larry Salkoff

Article

Wei et al Neuropharmacology. 1996 . 35(7):805-29.

Insert

Tandem pore domain K+ channel (twk-5 Nematode)

Use

Mammalian expression & oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pOX-twk24

Plasmid

#16120

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel (twk-24 Nematode)

Use

Xenopus oocyte expression

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pCM5.35

Plasmid

#19395

Depositor

Geraldine Seydoux

Article

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

nos-3 3'utr in pDONRP2RP3 (nos-3 Nematode)

Expression

Worm

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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mg172

Plasmid

#34652

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pBScMXT + egl2

Plasmid

#16209

Depositor

Larry Salkoff

Article

Weinshenker et al J Neurosci. 1999 Nov 15. 19(22):9831-40.

Insert

K+-channel KCNQ (egl-2 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only