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pAP639-2F

Plasmid

#99486

Purpose

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Expression

Bacterial

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP998-5

Plasmid

#99501

Purpose

3XFlag::tagBFP::linker::TEV

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::linker::TEV

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP973-1

Plasmid

#99496

Purpose

TEV::linker::meGFP:3XFlag

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::meGFP::3XFlag

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP576-2

Plasmid

#99481

Purpose

3XFlag::tagBFP::Myc

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::Myc

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP608-1

Plasmid

#99484

Purpose

H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP683-2

Plasmid

#99488

Purpose

TEV::eGFP::ER::HA::linker::3XFlag

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::HA::linker::3XFlag

Expression

Bacterial

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP684-3

Plasmid

#99489

Purpose

TEV::eGFP::ER::Ollas::linker::3XFlag

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::Ollas::linker::3XFlag

Expression

Bacterial

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP607-2

Plasmid

#99483

Purpose

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Publication

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Expression

Bacterial

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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AP1297‐1

Plasmid

#87247

Purpose

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Depositor

Geraldine Seydoux

Publication

Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)

Tags

gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBN237

Plasmid

#86719

Purpose

Tissue-specific FLP expression plasmid for C. elegans

Depositor

Peter Askjaer

Publication

Munoz-Jimenez et al Genetics. 2017 Aug;206(4):1763-1778. doi

Insert

Pnhr-82::FLP D5

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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oma-2 ORF in L4440

Plasmid

#82548

Purpose

oma-2 RNAi feeding construct

Depositor

Sean Ryder

Publication

Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

Insert

oma-2 ORF

Expression

Worm

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only
pMALac-OMA-1(1-182)

Plasmid

#80350

Purpose

Expresses OMA-1 (1-182) in bacteria for protein purification purposes

Depositor

Sean Ryder

Publication

Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

Insert

OMA-1 (oma-1 Nematode)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

Encodes AA residues 1-182

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1806_CRISPR_unc-73_exon21

Plasmid

#82360

Purpose

to create unc-73E null allele

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJL1604

Plasmid

#78194

Purpose

Ce cha-1 expression in motor neurons in worms

Depositor

Junho Lee

Publication

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

unc-3

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pJL1121

Plasmid

#78188

Purpose

intestinal GFP expression by fasting in worms

Depositor

Junho Lee

Publication

Jo et al Cell Metab. 2009 May;9(5):440-8. doi: 10

Insert

fil-2 (fil-2 Nematode)

Expression

Worm

Promoter

fil-2

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pClneoEGFPC RSF-1

Plasmid

#66862

Purpose

Express GFP-fused C.elegans RSF-1 in mammalian cells

Depositor

Yutaka Hata

Publication

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RSF-1 (rsf-1 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

S18G, S229T, I403T and F563S mutations in RSF-1 c…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pFLAG-WTS-1

Plasmid

#66856

Purpose

Express FLAG-tagged C.elegans WTS-1 in mammalian cells

Depositor

Yutaka Hata

Publication

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

WTS-1 (wts-1 Nematode)

Tags

FLAG

Expression

Mammalian

Mutation

E153G and D871G mutations in WTS1 compared to Gen…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
RGS-6xHis-BLMP-1-pcDNA3.1-

Plasmid

#52514

Purpose

expresses C. elegans BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

blmp-1 (blmp-1 Nematode)

Tags

6xHis

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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