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We narrowed to 4,128 results for: pCMV mammalian

Showing: 1961 - 2000 of 4128 results

  25. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322ā€¦
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  26. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid
    #185919
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322ā€¦
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185919-plasmid-map-sequence-id-367356

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  27. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and ā€¦
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET iā€¦
    Promoter
    CMV
    Available Since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    41732-plasmid-map-sequence-id-210671

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  29. pCMV-TNT-CFP-STIM1-C227W

    Plasmid
    #68403
    Purpose
    Expresses a STIM1 gain-of-function mutant in mammalian cells
    Depositor
    Yubin Zhou
    Article
    Ma et al Nat Commun. 2015 Jul 17;6:7826. doi: 10.
    Insert
    CFP-STIM1 (STIM1 Human)
    Tags
    Cyan Fluorescent Protein (CFP)
    Expression
    Mammalian
    Mutation
    Changed Cysteine 227 to Tryptophan; CFP was inserā€¦
    Promoter
    CMV
    Available Since
    Nov. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  30. pCMV-BRAF-REG-T241P-Halo

    Plasmid
    #202549
    Purpose
    Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains the RASopathy mutation, T241P, within its cysteine-rich domain.
    Depositor
    Deborah Morrison
    Article
    Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e
    Insert
    BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Threonine 241 mutated to proline
    Promoter
    CMV
    Available Since
    July 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    202549-plasmid-map-sequence-id-401163

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Showing: 1961 - 2000 of 4128 results