We narrowed to 32,226 results for: Eng

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pGEL637

Plasmid

#189832

Purpose

The T-DNA backbone of vCas12j2 for genome editing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL639

Plasmid

#189833

Purpose

The T-DNA backbone of Cas12j2 for DNA methylation-based gene silencing in rice

Depositor

Yong Zhang

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR; T-dna clone

Promoter

ZmUbi1

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA326

Plasmid

#214743

Purpose

High copy glmS-nadE plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV014

Plasmid

#214740

Purpose

High copy thyA-infA plasmid containing chloramphenicol as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV013

Plasmid

#214739

Purpose

Medium copy thyA-infA plasmid containing spectinomycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pSV015

Plasmid

#214738

Purpose

Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pKE011

Plasmid

#207912

Purpose

Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCS018

Plasmid

#207911

Purpose

Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS017

Plasmid

#207909

Purpose

Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS020

Plasmid

#207915

Purpose

Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKE012

Plasmid

#207914

Purpose

Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pKE010

Plasmid

#207910

Purpose

Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU3-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS019

Plasmid

#207913

Purpose

Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

Depositor

Alexis Maizel

Article

Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

Insert

pU6-AarI-scaffold-term

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL667

Plasmid

#196815

Purpose

LrCas9 genome editing plasmid in wheat

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL668

Plasmid

#196816

Purpose

LrCas9 genome editing plasmid in larix

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

Lrk004P

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL669

Plasmid

#196817

Purpose

LrCas9 genome editing plasmid in tomato

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

SlEF1a

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEL670

Plasmid

#196818

Purpose

LrCas9 cytosine base editing plasmid in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL671

Plasmid

#196819

Purpose

LrCas9 cytosine base editing plasmid in wheat

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL672

Plasmid

#196820

Purpose

LrCas9 adenine base editing plasmid V1.0 in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL673

Plasmid

#196821

Purpose

LrCas9 adenine base editing plasmid V2.0 in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL676

Plasmid

#196823

Purpose

dLrCas9 gene expression activation plasmid in rice

Depositor

Yong Zhang

Article

Zhong et al Nat Commun. 2023 Sep 29;14(1):6102. doi:

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-GFP-mSOX17

Plasmid

#206379

Purpose

Expresses EGFP fused mouse SOX17 in bacteria, for protein production.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Tags

EGFP

Expression

Bacterial

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-GFP-mSox17FNV

Plasmid

#206372

Purpose

Expresses EGFP fused mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Lentiviral

Tags

EGFP

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-mSox17FNVC2

Plasmid

#206370

Purpose

Expresses mouse SOX17FNVC2 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNVC2 (Sox17 Mouse)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-mSox17FNV

Plasmid

#206368

Purpose

Expresses mouse SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE2-TetO-mSox17

Plasmid

#206367

Purpose

Expresses mouse SOX17 in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17 (Sox17 Mouse)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-GFP-mSOX17FNV

Plasmid

#206380

Purpose

Expresses EGFP fused mouse SOX17FNV in bacteria, for protein production.

Depositor

Ralf Jauch

Article

Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

Insert

Sox17FNV (Sox17 Mouse)

Tags

EGFP

Expression

Bacterial

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131-RZ-Cas12j2

Plasmid

#189782

Purpose

Golden Gate entry vector to clone the 1st Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132-RZ-Cas12j2

Plasmid

#189783

Purpose

Golden Gate entry vector to clone the 2nd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ134-RZ-Cas12j2

Plasmid

#189785

Purpose

Golden Gate entry vector to clone the 4th Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ131-RZ-Cas12j2

Plasmid

#173927

Purpose

Golden Gate entry vector; empty vector to clone the 1st Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ132-RZ-Cas12j2

Plasmid

#173928

Purpose

Golden Gate entry vector; empty vector to clone the 2nd Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ133-RZ-Cas12j2

Plasmid

#173929

Purpose

Golden Gate entry vector; empty vector to clone the 3rd Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ134-RZ-Cas12j2

Plasmid

#173930

Purpose

Golden Gate entry vector; empty vector to clone the 4th Cas12j2 crRNA with ribozyme processing

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ265K

Plasmid

#173997

Purpose

Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

Insert

UNG-rAPOBEC1(R33A)-zCas9(D10A)

Use

CRISPR

Tags

3X FLAG, NLS

Expression

Plant

Mutation

R33A in rAPOBEC1 and D10A in zCas9

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pIALD2E

Plasmid

#185866

Purpose

Knocking out ALD6

Depositor

Claudia Vickers

Article

Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

Insert

ALD(-125, 40)- ALD6(1054, 1749)

Expression

Yeast

Mutation

None

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS-Clover4-LUMABS-DEN1

Plasmid

#183487

Purpose

To express the Clover4-LUMABS-DEN1 sensor for Dengue Virus antibody dection.

Depositor

Jun Chu, Zongwen Jin

Article

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-DEN1

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID5WY

Plasmid

#165050

Purpose

Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

OsTIR1 is codon-optimised for expression in S. ce…

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ290-D977A

Plasmid

#129674

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

D977A; AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ290-E848A

Plasmid

#129675

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Tags

E848A; AacCas12b is rice codon optimized

Expression

Plant

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ291-D573A

Plasmid

#129676

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b

Use

CRISPR; Gateway compatible bthcas12b entry clone

Expression

Plant

Mutation

D573A; BthCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ291-D951A

Plasmid

#129677

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b

Use

CRISPR; Gateway compatible bthcas12b entry clone

Expression

Plant

Mutation

D951A; BthCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ291-E827A

Plasmid

#129678

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b

Use

CRISPR; Gateway compatible bthcas12b entry clone

Expression

Plant

Mutation

E827A; BthCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ292-D977A

Plasmid

#129680

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D977A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ292-E848A

Plasmid

#129681

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

E848A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBTK224

Plasmid

#110590

Purpose

BTK Type 3 coding sequence for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Synthetic Biology

Available Since

Feb. 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pYPQ141-ZmUbi-RZ-Aa1.2

Plasmid

#136373

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141-ZmUbi-RZ-Aa3.8.3

Plasmid

#136374

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141-ZmUbi-RZ-Aac.3

Plasmid

#136377

Purpose

Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ141-ZmUbi-RZ-Aa1.2.3

Plasmid

#136372

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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