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pHHYTK128

Plasmid

#200140

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Ubc6-TA (233-250)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK127

Plasmid

#200139

Purpose

Type 4a part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

Fox2-PTS1 (870-900)

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB302-er-mCherry

Plasmid

#186287

Purpose

Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

er-mCherry

Expression

Bacterial

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pHHYTK123

Plasmid

#200130

Purpose

Type 7 part for Modular Cloning in Yeast

Depositor

Johannes Herrmann

Article

Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

Insert

pRS CEN/ARS

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4E-siRNAres_G

Plasmid

#146391

Purpose

Insect Expression of DmeIF4E-siRNAres

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmeIF4E-siRNAres

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-GST-V5His6_E

Plasmid

#146223

Purpose

Insect Expression of GST

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

GST

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-delta_E

Plasmid

#146181

Purpose

Insect Expression of DmAdh

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-PTC64-V5His6_E

Plasmid

#146183

Purpose

Insect Expression of DmAdh-PTC64

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh-PTC64 (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-DmAdh-V5His6_E

Plasmid

#146184

Purpose

Insect Expression of DmAdh

Depositor

Elisa Izaurralde

Article

Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

Insert

DmAdh (Adh Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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NarCas9_AAV

Plasmid

#192145

Purpose

Expresses NarCas9?and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

NarCas9

Use

AAV

Expression

Mammalian

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A-ZebrafishSTING

Plasmid

#196633

Purpose

Express Zebrasfish STING

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

ZebrafishSTING (sting1 Zebrafish)

Use

Lentiviral

Tags

mtagBFP

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pET29b-pvdF WT

Plasmid

#187974

Purpose

Expresses hyrdoxyornithine transformylase PvdF from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Kenjic et al Arch Biochem Biophys. 2019 Mar 30;664:40

Insert

pvdF WT

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-p450-hcvs-B2AD2-26Sfur-SEAP

Plasmid

#179647

Purpose

HCVP-inducible p450 SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-SEAP-26Sfur-B2AD-SpyTag-tvmvs-KKMP

Plasmid

#179648

Purpose

TVMVP-inducible single TM SEAP RELEASE construct with SpyTag

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-GFP-ECL-3TM-hcvs-KKMP

Plasmid

#179641

Purpose

HCVP-inducible membrane-bound GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Membrane-bound GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26sFur-B2AD-hcvs-tevs-KKMP

Plasmid

#179643

Purpose

OR Gate (TEVP/HCVP) SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pXCX-sGFAP-DLDH-IRES-tdTomato

Plasmid

#178311

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in astrocytes

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available Since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-miRFP670-Zdk1-MCS

Plasmid

#178873

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of miRFP670-Zdk1

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

miRFP670-Zdk1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAG.MCAK-mCherry-eDHFR

Plasmid

#176204

Purpose

MCAK (179-583) recruitment responsible for targeted microtubule depolymerization using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

MCAK-mCherry-eDHFR

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP925

Plasmid

#167591

Purpose

Express TrpC-TonB fusion protein

Depositor

Kathleen Postle

Article

Skare et al J Bacteriol. 1989 Aug;171(8):4442-7. doi

Insert

trpC-tonB

Expression

Bacterial

Mutation

Residues 1-25 TrpC; Residues 26-31 Linker; Residu…

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP933

Plasmid

#167657

Purpose

Express colicinB-His6, V21C, M261C

Depositor

Kathleen Postle

Article

Skare et al J Bacteriol. 1989 Aug;171(8):4442-7. doi

Insert

cba-his6 V21C, M261C

Expression

Bacterial

Mutation

cba-his6 with V21C, M261C mutations

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
PHY55-mouse U6-sgLMNA

Plasmid

#164045

Purpose

U6-driven sgRNA targeting LMNA

Depositor

Baohui Chen

Article

Xu et al Nucleic Acids Res. 2020 Oct 26. pii: 594

Insert

LMNA targeting sgRNA

Use

CRISPR

Promoter

mouse U6

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR 221-PHL2 (STOP)

Plasmid

#118683

Purpose

Gateway entry vector

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Other

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR 221-XERICO (STOP)

Plasmid

#118685

Purpose

Gateway entry vector

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Use

Other

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 527-PHL2

Plasmid

#118670

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 527-XERICO

Plasmid

#118671

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 544-PHL2

Plasmid

#118672

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Tags

His6-NusA

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 544-XERICO

Plasmid

#118673

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Tags

His6-NusA

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 565-PHL2

Plasmid

#118674

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Tags

His6-GST

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 565-XERICO

Plasmid

#118675

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Tags

His6-GST

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 566-PHL2

Plasmid

#118676

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST 566-XERICO

Plasmid

#118677

Purpose

Bacterial overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA cHA-FG1

Plasmid

#118665

Purpose

Plant overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

AT3G07200 (AT3G07200 Mustard Weed)

Tags

HA

Expression

Plant

Promoter

CaMV35S

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA cHA-XERICO

Plasmid

#118666

Purpose

Plant overexpression

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

XERICO (XERICO Mustard Weed)

Tags

HA

Expression

Plant

Promoter

CaMV35S

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_P0826S-sgRNA500

Plasmid

#149618

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0826S

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_PresTL-sgRNA500

Plasmid

#149617

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PresTL

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2H_Psyn-sgRNA500

Plasmid

#149559

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2_Psyn-sgRNAmreB

Plasmid

#149634

Purpose

mreB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2_Psyn-sgRNArodA

Plasmid

#149635

Purpose

rodA gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_Psyn-sgRNA500

Plasmid

#149614

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_PresTS-sgRNA500

Plasmid

#149616

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PresTS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_P0826L-sgRNA500

Plasmid

#149619

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0826L

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_P0526-sgRNA500

Plasmid

#149621

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_P0031-sgRNA500

Plasmid

#149622

Purpose

gRNA expression in B. burgdorferi, parental vector

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

P0031

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_PflaBS-sgRNAflaB

Plasmid

#149623

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaBS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_PresTS-sgRNAflaB

Plasmid

#149624

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PresTS

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_PresTL-sgRNAflaB

Plasmid

#149625

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PresTL

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSV2G_P0826S-sgRNAflaB

Plasmid

#149626

Purpose

flaB gRNA expression in B. burgdorferi

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

P0826S

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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