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Showing: 981 - 1000 of 5095 results

  1. pDisplay-GRAB_VIP1.0

    Plasmid
    #208682
    Purpose
    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208682-plasmid-map-sequence-id-419240

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  2. pDisplay-GRAB_NPYmut

    Plasmid
    #208678
    Purpose
    Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208678-plasmid-map-sequence-id-412165

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  5. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid
    #112029
    Purpose
    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    Use
    Tags
    Split mCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112029-plasmid-map-sequence-id-213508

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  6. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pgdh and Pxyl
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  7. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid
    #128606
    Purpose
    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Aug. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    128606-plasmid-map-sequence-id-254156

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  9. pAAV-hSyn-DIO-GRAB_VIP1.0

    Plasmid
    #208680
    Purpose
    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in a cre-dependent manner
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    208680-plasmid-map-sequence-id-412589

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  10. pAAV-hSyn-GRAB_VIP1.0

    Plasmid
    #208681
    Purpose
    Expresses the genetically-encoded fluorescent vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0 in neurons
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based vasoactive intestinal peptide (VIP) sensor GRAB_VIP1.0
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    208681-plasmid-map-sequence-id-410348

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  11. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid
    #171090
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Article
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…
    Expression
    Mutation
    D9A, H599A
    Promoter
    TUB1 (Toxoplasma gondii), transcriptionally linke…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171090-plasmid-map-sequence-id-335786

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  12. Negative Control Binder

    Plasmid
    #179140
    Purpose
    Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9) and an additionional A70Q mutation greatly reduces affinity for SsrA peptide
    Depositor
    Klaus Hahn
    Article
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Insert
    SspB
    Use
    Tags
    FLAG and mCherry
    Expression
    Mammalian
    Mutation
    Y11K/A15E, residue numbering from pdb: 1ou9. Addi…
    Promoter
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179140-plasmid-map-sequence-id-353410

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  14. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-Etanercept

    Plasmid
    #182424
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-Etanercept fusion proteins
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-mNeongreen-Etanercept (TNFRSF1B Human)
    VNp-LZ
    Use
    Tags
    Vesicle Nucleating peptide (VNp), and Vesicle Nuc…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182424-plasmid-map-sequence-id-360472

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  15. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

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Showing: 981 - 1000 of 5095 results