We narrowed to 4,504 results for: POR C
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Plasmid#186407PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only
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pENT-L1-Kozak-lacZ-stop-L2
Plasmid#186359PurposeEntry clone with ORF encoding lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of lacz reporterTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GFP-AURKA_K162M-mCherry
Plasmid#99881PurposeExpression of GFP-AURKA K162M-mCherry for protein purification in bacteriaDepositorInsertAURKA (AURKA Human)
UseTagsGFP and mCherryExpressionBacterialMutationK162M (kinase-dead)PromoterAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
cyt-GafD-mTurboID-V5
Plasmid#184640PurposeExpresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome taggingDepositorInsertcyt-GlycoID-V5
UseTagsNuclear export sequence and V5 tagExpressionMammalianMutationGafD -> expressed aa22-178 ; BirA* -> expre…PromoterCMVAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-UAP56 MUT
Plasmid#110124PurposeExpresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activityDepositorInsertUAP56 (DDX39B Human)
UseTagsFlagExpressionInsectMutation4 mutations (KSLN motif changed to RSFS)PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 MUT
Plasmid#110126PurposeExpresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activityDepositorInsertURH49 (DDX39A Human)
UseTagsFlagExpressionInsectMutation4 amino acid mutations (RSFS motif changed to KSL…PromoterMetallothionein Promoter (pMT)Available sinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2
Plasmid#182347PurposeGFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2
Plasmid#182349PurposemScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK2067
Plasmid#171845PurposeC. elegans mex-5 promoter (germline) driven dual fluorescent reporter for boxB tethering (eGFP) with boxB negative control (mCherry)DepositorInserthis-58, eGFP, mCherry
UseTagsN-terminal Tag OLLAS, V5 and C-terminal Tag PEST…ExpressionWormMutationboxB wild type and hairpin mutantPromotermex-5 promoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR-MRE16+MRE153-mut
Plasmid#178910Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationCTG>AGT and TGC>CAT mir16+153 mutatedPromoterSV40 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)
Plasmid#215759PurposeHDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)DepositorUseTagsIgG1 CH3ExpressionBacterialMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC2A11
Plasmid#132079PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A11 (SLC2A11 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
FlucWT-HA-GFP11-N1
Plasmid#91954PurposeExpresses FlucWT-HA-GFP11 in mammalian cellsDepositorInsertFlucWT-HA-GFP11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-HA-GFP11-N1
Plasmid#91953PurposeExpresses GST-HA-GFP11 in mammalian cellsDepositorInsertGST-HA-GFP11
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-Venus-Flag (865)
Plasmid#40998DepositorTypeEmpty backboneUseTags3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAG499-FRB-N-frFAST
Plasmid#160059PurposeExpresses FRB-N-frFAST in mammalian cellsDepositorInsertFRB-N-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMB63
Plasmid#47945PurposeExpresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromotereef-1A.1 (eft-3)Available sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only