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pcihygroflagsynhDAT

Plasmid

#19990

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

Dopamine transporter (SLC6A3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1wt-PA-mCherry

Plasmid

#138252

Purpose

Wild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1-PAmCherry

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-DAT R60A

Plasmid

#90247

Purpose

Expresses YFP-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Ma et al Sci Rep. 2017 Jul 14;7(1):5399. doi: 10.

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

mutated R60 to A in human DAT cDNA

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT W63A

Plasmid

#90246

Purpose

Expresses YFP-HA-DAT W63A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated W63 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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YFP-HA-DAT R60A

Plasmid

#90245

Purpose

Expresses YFP-HA-DAT R60A mutant in mammalian cells

Depositor

Alexander Sorkin

Article

Sorkina et al J Neurosci. 2009 Feb 4;29(5):1361-74. do

Insert

DAT (dopamine transporter) (SLC6A3 Human)

Use

Tags

Expression

Mammalian

Mutation

inserted HA tag (see YFP-HA-DAT), mutated R60 to A

Promoter

CMV

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pXD70EN20-Pct5-GPA_25810-(+)-Eldh1(GpDSM19378)

Plasmid

#225306

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70EN21-Pct5-GPA_25800-(+)-Eldh2(GpDSM19378)

Plasmid

#225307

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF25-Pct3-(–)-Eddh(nHis6)(G3C)

Plasmid

#233873

Purpose

Expresses His-tagged Gordonibacter pamelaeae 3C (–)-Eddh with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(–)-Eddh

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
GST_LIM1224

Plasmid

#182041

Purpose

For expression of human paxillin LIM domains 1, 2, 2 and 4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1224 (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

LIM domains 1, 2, 2 and 4

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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GST_LIM1334

Plasmid

#182040

Purpose

For expression of human paxillin LIM domains 1, 3, 3 and 4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1334 (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

LIM domains 1, 3, 3 and 4

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2.5-THP-GFP

Plasmid

#80336

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Article

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Tags

Expression

Mutation

Promoter

rat Tyrosine hydroxylase

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV2.5-THP-GFP/WGA

Plasmid

#80337

Purpose

Fluorescent Reporter for Dopaminergic Neuron Differentiation

Depositor

Kwang-Soo Kim

Article

Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

Insert

rat TH promoter (Th Rat)

Use

AAV

Tags

Expression

Mutation

Promoter

rat Tyrosine hydroxylase

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEYFP-Mitotrap

Plasmid

#46942

Purpose

Expresses Mitotrap in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Mitotrap (MTOR Budding Yeast, Synthetic, Human, Aequorea victoria)

Use

Tags

This is a fusion between a yeast mitochondrial ta…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pXD70DA9-Pct5-DadhABC(A2)

Plasmid

#191632

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD80ENSF26-Pct3-(+)-Eldh1(nHis6)(GpDSM19378)

Plasmid

#233874

Purpose

Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1 (GPA_25810 Gordonibacter pamelaeae DSM 19378)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pXD70EN19-Pct5-GPA_25810/25800-(+)-Eldh1/2(GpDSM19378)

Plasmid

#225305

Purpose

Expresses Gordonibacter pamelaeae DSM 19378 (+)-Eldh1 and (+)-Eldh2 in Eggerthella lenta DSM 2243 with a cumate inducible promoter

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh1, (+)-Eldh2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD80ENSF27-Pct3-(+)-Eldh2(nHis6)(GpDSM19378)

Plasmid

#233875

Purpose

Expresses His-tagged Gordonibacter pamelaeae DSM 19378 (+)-Eldh2 with a cumate inducible promoter in Gordonibacter urolithinfaciens DSM 27213 for protein purification

Depositor

Emily Balskus

Article

Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

Insert

(+)-Eldh2 (GPA_25800 Gordonibacter pamelaeae DSM 19378)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pXD70Tet(DadH)-Pdadh-DadhABC(A2)

Plasmid

#191644

Purpose

E. coli - Eggerthella lenta shuttle plasmid (TetR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP_Lims (MBP_LIM1234)

Plasmid

#182042

Purpose

For expression of human paxillin LIM domains 1-4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM1234 (PXN Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

LIM domains 1-4 only

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GST_LIM234

Plasmid

#182039

Purpose

For expression of human paxillin LIM domains 2-4 with N-terminal GST fusion protein in E. coli.

Depositor

Vesa Hytönen

Article

Ripamonti et al Commun Biol. 2021 Mar 29;4(1):380. doi:

Insert

LIM234 (PXN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

LIM domains 2-4 only

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge