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We narrowed to 25,844 results for: nes

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Showing: 981 - 1000 of 25844 results

  3. pCAGGS_cyt-AKAR5

    Plasmid
    #114138
    Purpose
    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    AKAR5 with C-terminal nuclear export signal (NES)
    Expression
    Mammalian
    Available Since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    114138-plasmid-map-sequence-id-215467

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  6. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    89308-plasmid-map-sequence-id-425605

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  7. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  8. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139094
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dSpCas9
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139094-plasmid-map-sequence-id-273891

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Showing: 981 - 1000 of 25844 results