Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,181 results for: nes

Clear keywords
Showing: 981 - 1000 of 16181 results

  4. pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)

    Plasmid
    #231237
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells
    Depositor
    Michael Rape
    Publication
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    NES fused to the DNA-binding-domain of MYC (resid…
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231237-plasmid-map-sequence-id-459172

    Enlarge

  5. pNESx2-EGFP-C1-PABDx2-Spo20

    Plasmid
    #220082
    Purpose
    PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export
    Depositor
    Gerry Hammond
    Publication
    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Spo20 (SPO20 Budding Yeast)
    Tags
    X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…
    Expression
    Mammalian
    Mutation
    amino acids 51-91, tandem dimer
    Promoter
    CMV
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220082-plasmid-map-sequence-id-434081

    Enlarge

  6. pcDNA3.1(+)-RasAR(R89L)-NES

    Plasmid
    #205568
    Purpose
    RasAR negative-control mutant plasmid. Targeted to cytosol.
    Depositor
    Jin Zhang
    Publication
    Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RasAR(R89L)-NES (RAF1 Human, Synthetic)
    Tags
    Nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Arg 89 mutated to Leu in Raf1 RBD region.
    Promoter
    CMV
    Available since
    Oct. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    205568-plasmid-map-sequence-id-409421

    Enlarge

  8. pcDNA-NES-ZapCmR1.1

    Plasmid
    #59014
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. pcDNA3-ERKsub(TA)DD-Venus-NES

    Plasmid
    #84631
    Purpose
    Encodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ERKsub(TA)DD-Venus-NES
    Tags
    6xHis, Nuclear export signal (NES), T7 tag (gene …
    Expression
    Mammalian
    Mutation
    Mutated target Thr residue to Ala.
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    84631-plasmid-map-sequence-id-175151

    Enlarge

  16. pDN34

    Plasmid
    #61343
    Purpose
    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)
    Depositor
    Barbara Di Ventura, Roland Eils
    Publication
    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    GS linker-AsLov2-biNLS2 and PKIt NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    61343-plasmid-map-sequence-id-228919

    Enlarge

  18. nCaMK2rep

    Plasmid
    #239624
    Purpose
    CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Publication
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Synthetic, Rat)
    Use
    Cre/Lox and Lentiviral
    Tags
    Flag and myc
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239624-plasmid-map-sequence-id-476245

    Enlarge

  19. nCaMK2rep-S603A

    Plasmid
    #239625
    Purpose
    Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Publication
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Synthetic, Rat)
    Use
    Cre/Lox and Lentiviral
    Tags
    Flag and myc
    Expression
    Mammalian
    Mutation
    Changed serine 603 to Alanine
    Promoter
    hSyn
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239625-plasmid-map-sequence-id-476241

    Enlarge

  20. Ratiometric FPX sensor for ERK kinase activity

    Plasmid
    #60974
    Purpose
    For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.
    Depositor
    Robert Campbell
    Publication
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ddRFP A-WW domain-ERK substrate-ddFP B
    Tags
    ERK substrate is before ddFP copy B, WW domain is…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 981 - 1000 of 16181 results