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POT4-CYC1p-ycrtI-ADH1t

Plasmid

#65357

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

CYC1p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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LexAop2->Bxb1.STOP>myr::4xSNAPf

Plasmid

#87640

Purpose

For creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPf

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSicoR-UBE2D3i1

Plasmid

#59586

Purpose

Expression of shRNA against human UBE2D3

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

UBE2D3 (UBE2D3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pJC20Cerulean3-Cdc8

Plasmid

#99357

Purpose

Bacterial expression of Cerulean3 fused to the 5' of cDNA of the fission yeast tropomyosin, cdc8.

Depositor

Dan Mulvihill

Article

Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

Insert

cdc8 (cdc8 Fission Yeast)

Tags

Cerulean3 and His6-Gly3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pET3a pro-RegIIIg

Plasmid

#64934

Purpose

bacterial expression of full length mouse RegIIIgamma

Depositor

Lora Hooper

Article

Cash et al Protein Expr Purif. 2006 Jul;48(1):151-9

Insert

RegIIIg (Reg3g Mouse)

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab25_4

Plasmid

#26712

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab25 RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Canine Rab25 RNAi.

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
XE75 Beta-catenin Delta N-9 GFP

Plasmid

#16831

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

beta-catenin Delta N - 9 GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Expression

Bacterial

Promoter

T7/M13

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mRFP-Syx2

Plasmid

#35704

Depositor

Arie Horowitz

Article

Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Ep

Insert

Syx2 (Plekhg5 Mouse)

Tags

mRFP

Expression

Mammalian

Mutation

R205K

Promoter

CMV

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
YIp211-P30-His-pol30(K127R)-3'

Plasmid

#99547

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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P4U2C6T2-v1-2 (Pl-tetO1-lacI) (137p)

Plasmid

#70213

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

lacI

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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HS-H3.3A d436-GFP

Plasmid

#8697

Depositor

Kami Ahmad

Article

Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

Insert

H3.3A truncated (His3.3A Fly)

Tags

GFP

Expression

Insect

Mutation

Deletion of amino acids 4-36.

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-Kdrl-3p-FokI-KK

Plasmid

#35685

Depositor

Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

Kdrl-3p-FokI-KK

Use

Mrna production

Tags

HA

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
pCS2-Flag-Kdrl-5p-FokI-EL

Plasmid

#35684

Depositor

Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

Kdrl-5p-FokI-EL

Use

Mrna production

Tags

FLAG and Flag

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
p3E-CMVmin:GFPmem no-pA

Plasmid

#80824

Purpose

CMVmin-promoted GFPmem without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

CMV-GFPCAAX nopA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFLIP30

Plasmid

#65499

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-mCer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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POT4-TEF2p-ycrtI-ADH1t

Plasmid

#65356

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TEF2p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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140_pSKB3-GBD-LUMP

Plasmid

#65972

Purpose

Bacterial expression of GBD-LUMP protein, a sensor for GTP-bound Cdc42

Depositor

Gerard Marriott

Article

Hoepker et al Proc Natl Acad Sci U S A. 2015 Apr 30. p

Insert

LUMP

Tags

GBD (GTPase binding domain from ACK1; residues 44…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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SepOTSν

Plasmid

#68294

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EF-Sep21, 5xtRNA-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pSicoR-PLCE1i2

Plasmid

#59629

Purpose

Expression of shRNA against human PLCE1

Depositor

Miguel Ramalho-Santos

Article

Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

Insert

PLCE1 (PLCE1 Human)

Use

RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only