Addgene

We narrowed to 25,797 results for: sta

Showing: 9981 - 10000 of 25797 results

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pK RagA-RagC

Plasmid

#99649

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

Kuang Shen, David Sabatini

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

10x His tag, Poly arginine linker, and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFE-vcDAB

Plasmid

#133004

Purpose

pFE carrying the vcdabA2 ( locus_tag: CSW01_RS07960) and vcdabB2 genes (locus_tag: CSW01_RS07955)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

vcdabB
vcdabA

Use

Tags

Expression

Bacterial

Mutation

Promoter

tetR

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pXIP TGBRI K to R

Plasmid

#19150

Depositor

Joan Massague

Article

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

Transforming growth factor, beta receptor I (TGFBR2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K420R mutation

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA FLAG mTOR ST

Plasmid

#22999

Depositor

Jing Huang

Article

Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 7. ()

Insert

mTOR (MTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S2035T

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
UKNP2.5.1

Plasmid

#98213

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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mEos2-FilaminA-C-14

Plasmid

#57378

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

FilaminA (FLNA Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

D2626H

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pBabe-Neuroserpin

Plasmid

#58259

Purpose

Retroviral vector for expressing Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pRS424-URR

Plasmid

#65330

Purpose

high copy number plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRS414-URR

Plasmid

#65329

Purpose

CEN plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-MA-VN

Plasmid

#123283

Purpose

Mammalian expression plasmid for matrix domain of HIV-1 Gag fused to the N-terminal half of split fluorescent Venus (VN)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

Matrix

Use

Tags

VN: N-terminus of split Venus (a.a. V1–A154) I152…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-eSpCas9

Plasmid

#133370

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

eSpCas9

Use

Tags

Flag

Expression

Mammalian

Mutation

eSpCas9 mutations

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAtCas9-dead_2

Plasmid

#91166

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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FusionRed-PMP-N-10

Plasmid

#56131

Purpose

Localization: Peroxisomes, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

PMP (PXMP2 Human)

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
MDH1-PGK-FLAG-TAT-86-A

Plasmid

#35977

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "A" HIV clade

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 S498A

Plasmid

#32215

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Ser498Ala

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
Lenti_CRISPRi_sgNeg2

Plasmid

#166999

Purpose

Lentiviral expression of negative control sgRNA for CRISPRi knockdown

Depositor

Gian-Paolo Dotto

Article

Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

Insert

Negative control sgRNA 2

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSiren_EGFP-shHmga2#3

Plasmid

#122291

Purpose

Knockdown for mouse Hmga2

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Insert

Knockdown shRNA for mouse Hmga2 (Hmga2 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM60 FLAG NUFIP1 W40A

Plasmid

#110951

Purpose

expresses mammalian FLAG-NUFIP1 W40A

Depositor

David Sabatini

Article

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 W40A (NUFIP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

changed Tryptophan 40 to alanine

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pVRc11_987

Plasmid

#49733

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

AS11_987

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pLux

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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px330-RPL26 sgRNA2

Plasmid

#134642

Purpose

contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

RPL26 sgRNA2 (RPL26 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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