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pAdEasy 2-GFP beta-gal

Plasmid

#16407

Depositor

Bert Vogelstein

Article

He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

Type

Empty backbone

Use

Adenoviral

Expression

Mammalian

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pACYCDuet-1_Ec_rpoZ

Plasmid

#128837

Purpose

E. coli rpoz gene cloned between NcoI and HindIII sites of the first MCS of this Duet plasmid. Omega protein.

Depositor

Seth Darst

Article

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Insert

rpoZ (rpoZ E. coli)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-HIS-Tol2

Plasmid

#112486

Purpose

functional recombinant Tol2 protein from E.coli

Depositor

Stephen Ekker

Article

Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

Insert

Tol2 Transposase (tpase Synthetic)

Tags

HIS/6x

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-copGFP

Plasmid

#73030

Purpose

Express copGFP

Depositor

Kazuhiro Oka

Insert

copGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTM1_VP35_ZEBOV

Plasmid

#68121

Purpose

Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

Depositor

Elke Mühlberger

Article

Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

Insert

VP35 ZEBOV (VP35 )

Use

T7

Promoter

T7

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-C-CreintG

Plasmid

#69573

Purpose

One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

Insert

kozak consensus - C-CreintG

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-dCas9-GFP

Plasmid

#64106

Purpose

Expression of Sp dCas9-GFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Sp dCas9

Use

CRISPR and Lentiviral

Tags

sfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pET11a-Z-NGFP

Plasmid

#52734

Purpose

IPTG-inducible expression of Z-NGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

NGFP+Z-fusion

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 mCerulean LIC cloning vector (6E)

Plasmid

#30128

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCerulean

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pHYRSF1-02

Plasmid

#24765

Depositor

Hideo Iwai

Article

Muona et al Nat Protoc. 2010 . 5(3):574-87.

Insert

NpuDnaE-IntC

Tags

GB1 and His

Expression

Bacterial

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pBI-p73 wt/EGFP

Plasmid

#16545

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

p73 (TP73 Human)

Tags

HA

Expression

Mammalian

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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MYD88

Plasmid

#13092

Depositor

Ruslan Medzhitov

Insert

MYD88 (Myd88 Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209653

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-mIgkc

Plasmid

#127157

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), kappa constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Igkc (Igkc Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAPf-hYTHDF3

Plasmid

#155348

Purpose

Express full-length human YTHDF3 with a C-terminal SNAP-tag in mammalian cells

Depositor

Xiaowei Zhuang

Article

Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

Insert

YTHDF3 (YTHDF3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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Ai38

Plasmid

#34883

Depositor

Hongkui Zeng

Article

Zariwala et al J Neurosci. 2012 Feb 29;32(9):3131-41. d

Insert

CAG-Floxed GCaMP3

Use

Mouse Targeting

Promoter

CAG

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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pUASTB

Plasmid

#18944

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 attB

Expression

Insect

Available Since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pRSETB PKA Cat

Plasmid

#14920

Depositor

Susan Taylor

Article

Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-eGFP-NMHC-IIAdeltaIQ2

Plasmid

#35690

Depositor

Tom Egelhoff

Article

Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

Insert

MHC IIA (MYH9 Human)

Tags

EGFP and GFPGenotRW

Expression

Mammalian

Mutation

The coding segment for aa 804-834 (corresponding …

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
pMM131

Plasmid

#127214

Purpose

BeYDV replicon with constitutive Luc expression, AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, AtSTM, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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