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pT7-EGFP-C1-HsNot1iso1_1318-1605-IKGIRQtoDSYDAA_W

Plasmid

#147894

Purpose

Mammalian Expression of HsNot1iso1_1318-1605-IKGIRQtoDSYDAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_1318-1605-IKGIRQtoDSYDAA (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBGE2.0

Plasmid

#165987

Purpose

Multi-host shuttle vector that can be transferred by conjugation and replicate in S. meliloti, E. coli, S. cerevisiae, and P. tricornutum. Contains S. meliloti pSymB origin and Tet resistance.

Depositor

Bogumil Karas

Article

Brumwell et al PLoS One. 2019 Jun 17;14(6):e0206781. do

Inserts

RK2/RP4 origin of transfer
repA1B1C1
nourseothricin N-acetyl transferase
tetracycline resistance

Use

Synthetic Biology; Bacterial and yeast cloning; c…

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-AH in pmCherryN1

Plasmid

#187284

Purpose

Express IL2-AH-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH

Promoter

CMV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-AHA740D, E758K in pmCherryN1

Plasmid

#187285

Purpose

Express IL2-AH A740D, E758K-mCherry

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH A740D, E758K

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-IgG1

Plasmid

#187010

Purpose

Expression of human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

Human anti-SARS-CoV-2 S Protein Wuhan-Hu1 IgG1

Use

Lentiviral

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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CIRC

Plasmid

#186343

Purpose

Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)

Depositor

Giovanni Blandino

Article

Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi:

Insert

VEGFA (VEGFA Human)

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 TAPBPR-TM-TN6

Plasmid

#178650

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

E205K, R207E, Q209S, Q272S, switched transmembran…

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pT22iU66CriT(#387)

Plasmid

#184061

Purpose

cyclofen-inducible CRE activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Sinha et al Zebrafish. 2010 Jun;7(2):199-204. doi: 1

Insert

CRE-ERT2

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22UbGfpnE4PCh(#301)

Plasmid

#184066

Purpose

cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

Insert

eGFP-nls-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

Plasmid

#179433

Purpose

Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

coreHS4-TRE-cit-coreHS4-mch (DC)

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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3020_pETcon-SARS-CoV-2-RBD_N501Y

Plasmid

#184405

Purpose

yeast surface display of the SARS-CoV-2 Alpha variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Alpha Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Tags

HA and c-Myc

Expression

Yeast

Mutation

N501Y

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-CBP(1603-2678)

Plasmid

#183774

Purpose

Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

nej (nej Fly)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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HEpl016

Plasmid

#179991

Purpose

pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

SiCas12i

Tags

3xFlag

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-SNAP-CD4-bla

Plasmid

#179452

Purpose

Donor vector to knock in SNAPtag C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

SnapTag

Use

CRISPR

Tags

His/Flag

Promoter

None

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X

Plasmid

#148000

Purpose

Mammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-KGRQtoSYAA-shRNAres_W

Plasmid

#147901

Purpose

Mammalian Expression of HsNot1iso1-KGRQtoSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-KGRQtoSYAA-shRNAres (CNOT1 Human)

Tags

GFP (G4C mutation)

Expression

Mammalian

Mutation

NM_016284.4, K1426S, G1451Y, R1458A, Q1549A

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-BTLAicd (AA190-289, Y257F, Y282F)-TwinStrep

Plasmid

#180811

Purpose

Expression of GST fused human BTLA intracellular domain (AA190-289, Y257F, Y282F)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

GST-BTLAint (AA190-289, Y257F, Y282F)-TwinStrep (BTLA Human)

Expression

Bacterial

Mutation

BTLA (Y257F, Y282F)

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His10-BTLAicd (AA190-289)-TwinStrep

Plasmid

#180805

Purpose

Expression of 10*His tagged human BTLA intracellular domain (AA190-289)-TwinStrep

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAint (AA190-289)-TwinStrep (BTLA Human)

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSL008_phiC31_neo-5xTetO-pEF-H2B-mCitrine-5kb,lambda-pRSV-H2B-mCherry

Plasmid

#179426

Purpose

Dual fluorescent reporter construct with 5kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-5kb-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pMH010_phiC31_neo-5xTetO-pEF-H2B-mCitrine-1.2kb,lambda-pRSV-H2B-mCherry

Plasmid

#179427

Purpose

Dual fluorescent reporter construct with 1.2kb lambda spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

TRE-cit-1.2kb-mch

Expression

Mammalian

Promoter

pEF alpha, pRSV

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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