159,428 results

1. pCSC-LHX8-IRES-GBX1

   Plasmid

   #182235

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   LHX8-IRES-GBX1 (LHX8, GBX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTT5-hSDC1

   Plasmid

   #52326

   Purpose

   expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

   Depositor

   Gordon Laurie

   Article

   Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

   Insert

   SDC1 (SDC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R117Q mutation (R95Q according to numbering in Fi…

   Promoter

   CMV

   Available since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAW62.YY1.FKBP.knock-in.mCherry

   Plasmid

   #104370

   Purpose

   For knocking in FKBP F36V P2A mCherry onto YY1

   Depositor

   Richard Young

   Article

   Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

   Insert

   FKBP12(F36V) -P2A-mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2R

   Plasmid

   #167932

   Purpose

   lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-N-Puro-mNeon-ACTB

   Plasmid

   #207752

   Purpose

   Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-FLAG-CAMK2D-ED

   Plasmid

   #221700

   Purpose

   Lentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cells

   Depositor

   Derrick Sek Tong Ong

   Article

   Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

   Insert

   CAMK2D (CAMK2D Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   K43R, D136N

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR - EGFP sgRNA 1

   Plasmid

   #51760

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS and U6

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-29b(+)_His-CBD-SpEndoH

   Plasmid

   #218196

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

   Depositor

   Florence Pojer

   Insert

   EndoH
   

   Use

   Tags

   tufA-His-CBD

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 45-313

   Promoter

   T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV JMJD3 mutant

   Plasmid

   #21214

   Depositor

   Paul Khavari

   Article

   Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

   Insert

   JMJD3 (KDM6B Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   H1390A

   Promoter

   Available since

   June 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL715

    Plasmid

    #140385

    Purpose

    Expresses MphR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-MphR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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