We narrowed to 617 results for: spen

1. FUG-T2A-D252G

   Plasmid

   #140741

   Purpose

   Lentiviral vector to overexpress the autism-associated PTEN mutation D252G and GFP as two separate proteins

   Depositor

   Bryan Luikart

   Article

   Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

   Insert

   GFP-T2A-PTEND252G (PTEN Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   D252G

   Promoter

   hUbiC

   Available since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pgRNA-CKB

   Plasmid

   #73501

   Purpose

   gRNA expression vector (with mKate2)

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   mKate2-T2A-Bsd
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVS1-PDi-CRISPRn

   Plasmid

   #73500

   Purpose

   Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   Cas9
   rtTA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-NC-CRISPRi (Gen3)

   Plasmid

   #73499

   Purpose

   Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Insert

   dCas9-KRAB
   

   Use

   CRISPR

   Tags

   HA, KRAB, and NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically deactivated Cas9)

   Promoter

   CAG

   Available since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3 1 kb prom. + Enhancer CD274

   Plasmid

   #107005

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   N.B. There are two published SNPs in this region …

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABE-hygro-2xMyc-Rac1-WT

   Plasmid

   #128580

   Purpose

   For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

   Depositor

   Julian Downward

   Article

   Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

   Insert

   Rac1 (Rac1 Mouse)
   

   Use

   Retroviral

   Tags

   2xMyc epitope

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRIPZ Myc2-mouse TTP aa S52 S178 MUT

   Plasmid

   #107001

   Purpose

   Expresses epitope-tagged double-mutant murine TTP

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   tristetraprolin (Zfp36 Mouse)
   

   Use

   Tags

   Double Myc peptide epitope tag

   Expression

   Mammalian

   Mutation

   Changed Ser52 to Ala and Ser178 to Ala

   Promoter

   Available since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3-EF1aH-TAZ S89A

   Plasmid

   #179910

   Purpose

   Construct for expressing an activated form of TAZ (encoding be gene WWTR1)

   Depositor

   Xin Chen

   Article

   Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

   Insert

   WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
   

   Use

   Tags

   3X Flag-tag

   Expression

   Mammalian

   Mutation

   Serine 89 mutated to Alanine

   Promoter

   EF1a

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

   Plasmid

   #107009

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-noSV40-humanCOL2A1enhancer

    Plasmid

    #173961

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Enhancer region from COL2A1 gene
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    SV40 promoter

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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