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pcDNA3-Lyn-Venus-cpVenus-FLARE-AKAR

Plasmid

#123337

Purpose

Plasma membrane-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Lyn-Venus-cpVenus-FLARE-AKAR

Tags

N-terminal targeting sequence from Lyn kinase, Ve…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-CKAR

Plasmid

#123343

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCK669

Plasmid

#192638

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dxCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dxCas9-NG h…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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lentiGuideFB-Puro-B

Plasmid

#192507

Purpose

For cloning of sgRNAs compatible with PspCas9. Contains a 5' direct repeat and a unique sgRNA scaffold variant with a capture sequence. Cloning guide RNAs using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Methods. 2023 Jan;20(1):86-94. doi:

Insert

bU6-sgRNACR2-CS-BsmBI-EFS-Puro-WPRE

Use

Lentiviral

Promoter

bU6

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-Venus-FLARE-AKAR

Plasmid

#123331

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

Plasmid

#123342

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-EKAR-EV

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP2-WW1-mutant

Plasmid

#17795

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
p2xFLAGhYAP2-WW2-mutant

Plasmid

#17796

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 258 to Alanine Proline 261 to Alanine

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

Plasmid

#123341

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-EKAR-EV

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pM-ErbB4-delta-kinase-PY3 mutant

Plasmid

#17802

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pLIC-His6-MBP-TEV-Rgs14

Plasmid

#236395

Purpose

Expresses His6-MBP-TEV-Rgs14 in E. coli

Depositor

John Hepler

Article

Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Tags

hexahistidine (H6) tag and maltose-binding protei…

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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p40651-EFSNS-FMR1_e1ΔCGG-24xms2

Plasmid

#222969

Purpose

MS2 mRNA reporter with FMR1 5' UTR (CGG repeats removed) and exon 1 sequence

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1-ΔCGG 5' UTR and exon1

Expression

Mammalian

Mutation

31 CGG repeats deleted from 5' UTR

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1-49535-sgFmr1_CGG5-2

Plasmid

#222964

Purpose

Targeting FMR1 5' UTR for CGG deletion

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 sgRNA (FMR1 Human)

Use

CRISPR

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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T40A+Q54A

Plasmid

#217913

Purpose

A plasmid encoding the hydrogel-forming protein PXP with both the T40A and Q54A coil mutations

Depositor

Cole DeForest

Article

Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

Insert

PXP T40A+Q54A

Tags

6x His (N and C term)

Expression

Bacterial

Mutation

Threonine at position 41 and 253 mutated to Alani…

Promoter

T5

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-F14F15S-jGCaMP7s

Plasmid

#178514

Purpose

AAV vector for Flpo recombinase dependent jGCaMP7s

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

jGCaMP7s

Use

AAV

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-mCre

Plasmid

#178515

Purpose

AAV vector for mCre recombinase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCre

Use

AAV and Cre/Lox

Expression

Mammalian

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_DCUN1D1_PONY

Plasmid

#178473

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

DCUN1D1_PONY (DCUN1D1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER

Plasmid

#123354

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

cp-moxCerulean3-moxCerulean3-FLARE-D1ER

Tags

C-terminal ER retention signal (KDEL), cp-moxCeru…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

Plasmid

#123340

Purpose

Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-EKAR-EV(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-CKAR

Plasmid

#123345

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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