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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,138 results for: fret

Showing: 1001 - 1020 of 1138 results

  1. pKK-TEV-mVenus

    Plasmid
    #105791
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mVenus
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105791-plasmid-map-sequence-id-203382

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  3. beta catenin binding mutant of alpha catenin sensor

    Plasmid
    #71711
    Purpose
    This form of the alpha catenin sensor lacks the beta catenin binding site and does not localize to cadherin junctions
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin Δβ-cat mutant FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 48- 163
    Promoter
    CMV
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71711-plasmid-map-sequence-id-142367

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  5. VBS mutant of alpha catenin sensor

    Plasmid
    #71710
    Purpose
    This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.
    Depositor
    Deborah Leckband
    Article
    Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi
    Insert
    alpha-catenin ΔVBS FRET Sensor
    Tags
    ECFP and YPET
    Expression
    Mammalian
    Mutation
    Deleted amino acid residues 316-405 in α -catenin…
    Promoter
    CMV
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71710-plasmid-map-sequence-id-142366

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  6. cfmTurquoise-N

    Plasmid
    #139284
    Purpose
    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.
    Depositor
    Ikuo Wada
    Article
    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d
    Insert
    cfmTurquoise
    Tags
    Multi-cloning sites
    Expression
    Mammalian
    Mutation
    Cys48Ser and Cys70/Met
    Available Since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    139284-plasmid-map-sequence-id-291944

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  11. pCDNA3.1-nAChr-beta3

    Plasmid
    #48811
    Purpose
    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
    Depositor
    Jerry Stitzel, Henry Lester
    Article
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    Expression
    Mammalian
    Mutation
    *
    Promoter
    CMV
    Available Since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    48811-plasmid-map-sequence-id-193963

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  12. pKK-mVenus-TEV

    Plasmid
    #105774
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mVenus-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105774-plasmid-map-sequence-id-203363

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  14. pKK-TEV-mAmber

    Plasmid
    #105792
    Purpose
    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the C-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    TEV-mAmber
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105792-plasmid-map-sequence-id-203383

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  16. pKK-mAmber-TEV

    Plasmid
    #105775
    Purpose
    Expression of your protein of interest in fusion with non-absorbing, non-emitting Venus mutant at the N-terminus (cleavable by TEV). Negative control for FRET measurements (PMID: 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mAmber-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105775-plasmid-map-sequence-id-203364

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  18. pKK-BiFC-Venus

    Plasmid
    #105804
    Purpose
    Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    VN173 (I152L); VC155
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105804-plasmid-map-sequence-id-203400

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Showing: 1001 - 1020 of 1138 results